Pig artificial insemination technology, who can tell us all about it?

(agricultural get Rich Network) Pig artificial insemination technology, who has a comprehensive description? 1. Cleaning and disinfection of instruments: rinse once with 2% sodium bicarbonate (baking soda) solution. Rinse again with clean water for 5 or 6 times. Disinfection: glass, metal, gauze, towels are first boiled and then sterilized in a constant temperature drying box (120min, 15min); seminal tubes are sterilized by boiling or steam sterilization; water thermometer is sterilized by alcohol cotton ball; diluent is boiled for 10 minutes or boiled directly for 15 minutes, and sterilized instruments are washed with dilution before use. 2. The sanitary window of the operating room is clean, the console and floor need to be cleaned every day, and the operator should wear white overalls to make it dust-free, fly-free and sterile. absolutely no smoking. Instruments shall not be used in series or mixed with veterinary instruments and drugs. 3. To make a fake pig, a log with a diameter of 20 cm and a length of 110 cm to 120 cm is cut into an arc at both ends, then 4 legs are installed and fixed. Cover the wood with a layer of straw or straw bag and cover with a piece of cooked pig skin. The height of the hindquarters and forequarters of the assembled fake pigs is 55cm and 56cm, respectively, which is low in front and high behind, with a difference of 10 cm. 4. Training boars to apply urine and mucus from estrus sows on the hindquarters of fake Taiwan pigs to induce boars to climb across. Drive the estrus sow next to the boar, let the trained boar climb, drive the sow away when the boar sexual desire reaches the best part, and then lure the boar to climb the boar, and use the trained boar to climb the boar to watch, in order to stimulate its libido and induce it to climb across the sperm collection. Generally, after 3 or 5 times of successful training, it should be collected for several days after successful training, in order to consolidate its conditioned reflex, and the trained boars are no longer allowed to mate. 5. The sperm collection method should be carried out indoors. The semen collection room must be kept clean and dust-free, quiet without interference, and the ground flat and non-slippery. In order to prevent heat stroke caused by high temperature in summer, semen collection should be carried out in the morning, cold in winter, the indoor temperature of semen collection should be kept above 15 ℃, and the collecting cup should be provided with heat preservation facilities to prevent semen from reducing semen quality due to cold shock or repeated heating up many times. Manual extraction method: before collecting semen, cut off the long hair around the boar prepuce, fake Taiwan pig and breeder boar penis foreskin, under the abdomen and other places with 0.1% potassium permanganate solution to clean. The sperm collector should trim his nails and wipe off the volatiles with 70% alcohol. When the boar climbs onto the fake platform pig, according to the habit of the semen collector, he can squat on the left and right side of the fake pig. After the boar climbs across and twitches the penis several times, the semen collector quickly holds the penis downwards with the (left) palm of the right hand, and holds the front end of the penis with his thumb to prevent abrasions, and the tightness of the grip is to prevent the penis from slipping. The semen that boar begins to ejaculate is mostly semen, and mixed with urine and dirt, so it is not suitable to be collected. When the thicker milky semen is ejaculated, it is collected in the semen collecting cup. Generally, it can ejaculate 2 times and 3 times in the process of one semen collection. After the boar ejaculates, the semen collector should hand-feed the penis into the foreskin and avoid rough push or beating. Adult pigs collect semen every other day, sometimes once a day when insemination is tense, and once every 3 days for young pigs (1 year old) and old pigs (over 4 years old). 6. The temperature of semen quality inspection room should be kept above 15 ℃, and semen should be filtered with 3-4 layers of gauze. Check semen volume, color, smell, vitality and density. General ejaculation volume 200 million 500 ml, the normal color is milky white or grayish white, slightly fishy smell. During the examination, 1 drop of filtered semen (the size of sorghum grain) was dipped in a sterilized thin glass rod, and the point was placed on the carrier. The cover was covered and examined under a microscope of 250mm 400 times. The light should not be too strong, and the local temperature of semen examination should be 35 ℃ ~ 37 ℃. Sperm motility should not be lower than 0.7 in general. Semen at room temperature with activity less than 0.5 should not be used. Sperm density refers to the number of sperm per milliliter of semen, generally should not be less than 100 million motile sperm. 7. When diluting semen, all equipment and containers that are in direct contact with semen must be disinfected. The temperature should be consistent with the semen temperature. Rinse once with a small amount of isothermal diluent before use. Composition of diluent formula. Method 1: glucose 5g, sodium citrate dihydrate 0.3g, disodium diamine tetraacetate 0.1g, distilled water 100ml, penicillin 100000 units, dihydrostreptomycin 1000 μ g / ml, for preservation at room temperature. Method 2: glucose 5g, sodium citrate dihydrate 0.5g, distilled water 100ml, appropriate amount of penicillin and streptomycin. For storage at room temperature. Method 3: glucose 5g, sodium citrate dihydrate 0.5g, distilled water 100ml, fresh yolk 3ml, appropriate amount of penicillin. Used for room temperature preservation or ordinary cryopreservation. Diluent preparation method: weigh medicine and water with balance and measuring cylinder respectively, deeply dissolve, disinfect, add penicillin when cooling to about 30 ℃, filter with sterilized gauze 4 or 2 layers of silk or qualitative rapid filter paper, set aside. If the yolk is used, then add the yolk and mix it fully. Make it ready-to-use. Dilution requirements: dilution as early as possible after semen collection. The diluent should be equal to or slightly lower than the semen temperature, with a maximum difference of 1 ℃. The diluent is slowly poured into the semen along the water thermometer, glass rod or semen bottle wall, and the semen shall not be poured directly into the diluent. The proportion of semen dilution should be determined according to the quality of the original semen, the number of sows needed, and the need for transportation and storage, generally 1 ∶ 1 × 3 or according to the principle of 100 million motile sperm per milliliter of diluted semen, dense semen can be diluted 2 to 3 times, medium semen can be diluted 1 to 2 times, and dilute semen can only be diluted 0.5 times. 8. Semen storage and transportation storage methods: no cooling is required for storage within 3 to 4 hours. Just keep it at room temperature. After more than 5 hours of storage, the semen containers that have been naturally cooled can be sealed and stored in 15-20 ℃ incubators or flasks, which should be used up within 24 hours. Semen containers should be fully sealed and wrapped with towels to avoid foam during transportation, shorten transportation time as much as possible, and control temperature changes. In winter, the incubator is used to adjust the temperature in the box at about 20 ℃ ~ 25 ℃ to prevent sudden high and low. Direct sunlight and the smell of alcohol and tobacco should be avoided. 9. Insemination is based on the estrus performance of sows, vaginal mucosa examination and asking the animal owner to comprehensively determine the appropriate time for insemination. The sows usually inseminate for the first time 18-36 hours after estrus. The second insemination was performed in 36 hours and 48 hours. First-born sows usually inseminate for the first time in 36-48 hours, which should be based on the principle of early and late maturation. Each estrus was instilled 2 or 3 times. Before insemination, the vulva of sows was washed and dried with warm water, and the preserved semen was heated to more than 25 ℃ and less than 36 ℃ by using human body temperature or put into 38 ℃ water. Semen transfusion volume: it should be determined according to the body weight and semen quality of sows, sperm motility 0.6 / 50 kg body weight 15 20 mg. The sperm motility was more than 0.7 and 10-15 ml was infused every 50 kg body weight. Insemination method: the general sow does not need Baoding, open the vulva in one hand, insert the fine rubber tube in the other into the vagina, insert about 10 cm oblique above, then insert it horizontally, twirl and push it while inserting it. When you feel unable to insert about 30 cm, you can input semen. In order to prevent countercurrent, you can pat the waist after transfusion. It is better to send a single lap for an hour before feeding. The artificial insemination technology of pigs is imperative for intensive pig farms, and the majority of farmers also hope to master this technology, which is a good thing for boar operators. It can not only greatly reduce the number of breeding boars, improve economic benefits, but also effectively reduce sow reproductive diseases.