Bacillus subtilis freeze-drying agent

In recent years, with the concept of healthy and pollution-free aquaculture deeply rooted in the hearts of the people, the control of aquaculture water quality is becoming more and more important, and microbial preparations are more and more concerned and welcomed by the majority of aquaculture. However, how to make microecological agents really play a role is what we have always been concerned about and concerned about. At present, the emergence of freeze-dried bacteria of Bacillus and yogurt has solved the problems in the use of microecological agents, such as many miscellaneous bacteria, low content of living bacteria, aging of original bacteria and so on.

1. Characteristics of living bacteria liquid cultured with bacteria

1. High purity: vacuum bottle packaging is adopted to avoid external contamination of miscellaneous bacteria.

2. High content: the concentration of living bacteria cultured with freeze-dried bacteria can be as high as 10 billion cfu/ml, and the normal concentration is not less than 5 billion cfu/ml, which is much higher than that of ordinary microecological agents on the market.

3. Strong activity: the current living bacterial liquid ensures the freshness of the bacteria, thus avoiding the bacterial aging phenomenon caused by long-term storage, and fully ensuring the activity of beneficial bacteria.

Second, the experiment of cultivating live bacteria solution with bacteria.

The main results are as follows: 1. The strain culture medium is prepared with brown sugar, and the addition ratio of water to brown sugar is 10:1, that is, 10 jin of water and 1 jin of brown sugar, which can basically meet the needs of strain reproduction and growth. After numerous experiments of strain diffusion culture, the growth curve shown in the following figure is obtained. From the growth curve of the two strains, it is easy to see that the strain culture medium has the best effect when it is used between 24 and 48 hours, so our strain should be cultured for 2 days before use.

2. In the process of cultivating live bacteria solution with bacteria, sampling observation and counting are carried out in different time periods, as shown in the following figure:

3. Comparison between strain culture medium and common microecological preparation (as follows):

The living bacteria solution cultured by bacteria has many advantages over ordinary microecological agents, whether it is the freshness of beneficial bacteria, the content of living bacteria, the activity of living bacteria and so on.

IV. Main functions of freeze-dried bacteria

The function of ㈠ Bacillus

1. Decompose the excreta, residual bait and organic dirt of aquatic animals, eliminate the odor of the water body, purify the water quality and improve the transparency.

2. Rapid degradation of toxic substances such as nitrite, ammonia nitrogen and hydrogen sulfide to inhibit the reproduction of blue-green algae.

3. This product is often used in greenhouse and greenhouse culture, which can effectively purify the water body, alleviate the problems caused by inconvenient water exchange and difficult ventilation, and improve the success rate of culture.

4. It is suitable for seawater and freshwater aquaculture water with all kinds of salinity and pH.

The function of freeze-drying Agent ㈡

1. Recuperate and improve the intestinal tract of aquatic animals, which has the function of preventing and treating enteritis.

2. Improve the disease resistance of aquatic animals and promote the rapid growth of aquatic animals.

3. It has strong tolerance to many kinds of antibiotics, and its biological effect will not be affected when combined with antibiotics.

4. It belongs to non-aerobic bacillus, which can purify and worsen the water quality of the pond and play an anti-stress role.