MySheen

Inoculation technique of Auricularia auricula

Published: 2024-11-05 Author: mysheen
Last Updated: 2024/11/05, 1. The fog irrigation facility is composed of dry, branch and wool high pressure polyethylene plastic pipe micro atomization nozzles and pipe fittings. The diameter of the main pipe is 40ml 50mm, the diameter of the branch pipe is 25mm 12mm, and the diameter of the capillary tube is 10mm 12mm. During installation, the water source of the main pipe is perpendicular to the branch pipe, the branch pipe is perpendicular to each ear trunk, and the capillary (usually 15.

After sterilization, the plastic bag is cooled for 2030 ℃, then packed in the inoculation box (room), sealed with 20.3% Lysu water spray for half an hour, or illuminated with ultraviolet light for half an hour. Then select the cultivated species with exuberant mycelium. The strain should be selected strictly, and when it is confirmed that it is not contaminated by miscellaneous bacteria, wipe the outside of the seed bottle thoroughly with alcohol cotton balls and put it into the inoculation box (room), and mash the cultivated seeds with sterilized tweezers. In the aseptic area of the lit alcohol lamp, pour 5 to 6 grams of cultivated seeds into a plastic bag filled with ingredients, and then plug the mouth of the plastic bag with cotton plugs and Kraft paper as they were before inoculation. the operation should be quick and agile to reduce the chance of infection of miscellaneous bacteria.

 
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