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What are the steps in the cultivation of hericium Erinaceus?

Published: 2024-11-23 Author: mysheen
Last Updated: 2024/11/23, What are the steps in the cultivation of hericium Erinaceus? Please introduce the first is the production of bacteria: strains can be divided into mother species, original species and cultivated species. Mother seed is preserved in the test tube slope of the bacteria, the production is more strict, generally preserved in scientific research institutions or conditional production units. Individuals or professional households can ask for small production from these units.

What are the steps to cultivation of Hericium erinaceus? Please give an introduction One is to make strains: strains have mother species, original species and cultivated species of points. Mother species is preserved in the test tube slant strains, production is more strict, generally preserved in Scientific research institutions or qualified production units. small quantities produced by individual or professional households can be obtained from these units. Second, the original seed is expanded from the mother seed. Its raw materials are hardwood sawdust 75%, wheat bran 23%, sugar 1%, gypsum or calcium carbonate 1%. Weigh the above raw materials according to the production quantity, add water and stir evenly. Add water until you hold it tightly with your hands but it doesn't drip. Then culture material into cans, cans to be washed in advance, drying. While loading, compact it by hand, leaving a gap of 3~4 cm from the bottle mouth, and do not install it flat to the bottle mouth. Then use a stick as thick as a finger to make a hole in the bottle material, leading to the bottom of the bottle. Then wipe off the culture material attached to the bottle and the bottle mouth with a cloth, wrap the bottle mouth with a layer of plastic film, tie it tightly with rope or cowhide tendon, and sterilize. Third, there are two methods of sterilization, one is to use an autoclave or autoclave, sterilization for half an hour to 1 hour. If there is no pressure cooker, use a steamer, put the cans filled with culture materials one by one in the pot, cover the lid tightly, and maintain it for 1 ~2 hours after SAIC. For the purpose of complete sterilization, it is best to cook for about 1 hour every other night. Fourth, after sterilization, put the can bottle into the inoculation box. If there is no inoculation box, put it in a clean place. In front of the flame of an alcohol lamp, transfer a fingernail-sized slant of the strain with a strain inoculation needle into the hole in the middle of the can. This operation should be done quickly to avoid contamination of miscellaneous bacteria. After inoculation, the bottle opening is covered with plastic film, tied with rope, and cultured at constant temperature of 25 ~28℃. Generally, one slant strain can be connected with 5~7 bottles of original seed, and each can bottle inoculation needle should be burned once on the flame of alcohol lamp. After 20~30 days of culture, white hyphae can be seen growing all over the bottle. No light is needed at this stage and the air should be kept as fresh as possible. Fifthly, culture management: according to the culture medium formula and operation method of the original seed, the original seed is further expanded for one culture, that is, planting seed. Generally, 30~40 bottles of seed can be planted with one bottle of original seed, and the temperature and time of culture are basically the same as those of original seed. Click for more Hericium erinaceus cultivation techniques Click for more edible mushroom cultivation techniques

 
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