How to cultivate Pleurotus ostreatus with liquid strain?

How to cultivate Pleurotus ostreatus with liquid strain? Please introduce the liquid strain cultivation of Pleurotus ostreatus can refer to the following methods: 1. The optimum temperature for mycelial production of Pleurotus ostreatus is 22lle 26 ℃, and the suitable fruiting body growth is 18Mel 25 ℃. Because of the long cycle, the traditional cultivation methods can produce mushrooms from February to March in spring, from April to June, or from August to September in autumn, and from mid to late October and March of the following year. The cultivation period is greatly shortened by using liquid bacteria, because the liquid strains germinate quickly, eat food quickly, and the mycelium growth time is short. at the same time, many links of intermediate seed production are omitted, so it can be cultivated in many seasons every year, so that the economic benefit is doubled. 2. The reasonable preparation of culture medium has strong activity of mycelial protease, and rich nitrogen source is needed for growth and development. When preparing the culture medium, the addition amount of nitrogen source auxiliary materials should be appropriate, if the nitrogen content of the culture material is too high, it will cause high pollution rate and late mushroom emergence; if the nitrogen content of the culture material is too low, the yield will be affected. After repeated cultivation experiments, the researchers of our unit selected the following formula: (1) 63% of hard sawdust, 20% of cottonseed shell, 15% of wheat bran, 1% of gypsum and 1% of quicklime. (2) hard sawdust 75%, cottonseed shell 10%, wheat bran 10%, cornmeal 3%, calcium carbonate 1%, gypsum 1%. (3) sawdust 43%, corncob 39%, wheat bran 15%, cottonseed shell 10%, calcium carbonate 1%, gypsum 1%. 3. Choose fresh and mildew-free culture materials for mixing, bagging and sterilization, with moderate thickness. If the raw material is too coarse, it is easy to break the plastic bag, causing miscellaneous bacteria pollution; too fine affects the air permeability, and the mycelium grows slowly. Dry mix the prepared raw materials evenly, add water and adjust the water content to about 60%. When loading, 17 × 33 cm angle plastic bags are used, each bag contains 300ml 400g of dry material, covered with no cotton cover and sealed. Sterilization at atmospheric pressure for 100 ℃ was maintained for 8 hours, and liquid bacteria were added after cooling. 4. It takes only 3 days for liquid strain incubator to produce a batch of bacteria, which is 10% of the cycle of solid bacteria. The cost of each tank is 195 yuan, and 5000 bags can be received. Because of the fluidity of liquid bacteria, there are many germination points. 6 hours after inoculation, the bacteria can germinate and turn white, and after 24 hours, they can eat and grow, so that the miscellaneous bacteria have no chance of infection. Generally, under the condition of 25 ℃, the cultivation bag can be filled after 20 days, and 50% of the solid bacteria are used in the growing period. 5. Mushroom management tea tree mushroom is sensitive to light, it is easy to form fruiting body in the case of scattered light and variable temperature, and no light is needed in the stage of mycelial culture. After the tea tree mushroom mycelium grows full, it moves into the mushroom shed or unlocks the mouth of the bag to increase the air and scattered light, increase the temperature difference, spray water on the ground and space, so that the air humidity reaches 85%, and the mushroom temperature is controlled at 1026 ℃. In this range, if we can keep the high temperature (or low temperature) 3mi for 5 days, and then make the temperature suddenly drop (or rise) 3mi for 5 days, it will be beneficial to the formation of primordium, make the mushroom more orderly, the clump is larger, and the tide is obvious. If the mycelia on the surface of the bag become brown patches with white mycelium masses after 7 days of proper management, the primordium can be seen at this time and can be harvested in about 10 days. In order to reduce the moisture loss of the culture material, the mouth of the bag should not be too large when opening the bag, the cover can be removed, and the ring can be removed after the fruiting body is formed, and the mouth of the bag can be untied to produce mushrooms. After each harvest of Pleurotus ostreatus, it is necessary to scratch the dehydrated mycelium on the surface of 1-2cm and replenish water at the same time, so that the fruiting body of Pleurotus ostreatus is differentiated, the quantity is more and the yield is high. 6. prevention and control of diseases and insect pests in the mushroom emergence stage, the mushroom shed and its surrounding environment should be kept clean and sanitary. Before mushroom emergence, the mushroom shed can be disinfected with dichlorvos and carbendazim to reduce the source of bacteria and insects. In the management of mushroom production, attention should be paid to ventilation and ventilation to avoid the spread of diseases and insect pests caused by high temperature and humidity. Humidity should be properly controlled when mushrooms are produced in the high temperature season, less water should be sprayed, do not spray water on the mushroom body, and dead and rotten mushrooms should be picked and dealt with in time. 7. Pleurotus ostreatus should be harvested when the cover is hemispherical, and if harvested too late, the cover is easy to dry, break or fall off, and its commercial value is low. When harvesting, squeeze the base of the fungal stalk with your fingers and gently spin it up to make the mushroom body out of the culture medium. After removing the impurities in the root, the mushroom can be sold fresh or dried. Each bag can pick 3mur5 wet mushrooms, the bag can produce fresh mushrooms 0.2-0.3kg, and the bioconversion rate can reach about 70%. Click to get more tea mushroom planting technology click to get more edible mushroom planting technology