Mr. Lu Dexun would like to introduce some books on nutrition.

1. Molecular Nutrition-- the author of supporting textbooks for Colleges and Universities in China: edited by Zhang Changhao

Publisher: people's Health Publishing House

Publication time: 2006-7-1 words: 469000 editions: 1 page: 251Printing time: 2006-07-01 I S B N: 9787117077705 content introduction

The book is divided into five chapters. The first chapter briefly introduces the concept of gene expression and the basic theory of gene expression regulation, because the regulation of gene expression by nutrients involves these basic theories of molecular biology. therefore, the introduction in front of this book will help to lay a foundation for later knowledge. The second chapter is about the regulation of nutrients on gene expression and their effects on genome structure and stability, which is one of the main research contents of molecular nutrition. In the third chapter, taking various nutrients as an example, the regulation of gene expression by each nutrient is introduced in detail, because although nutrients have something in common, they have their own characteristics at the same time. The fourth chapter is the effect of gene polymorphism on the absorption, metabolism and utilization of nutrients, which is another main research content of molecular nutrition. On the basis of the above chapters, the fifth chapter discusses the law and mechanism of the interaction between nutrients and genes on health, that is, the role of the interaction between nutrients and genes in the occurrence of diseases. The content of this book is more, involving a lot of knowledge of molecular biology, with the main reference materials attached at the end of the text, which can be used as a reference for students' further reading and teachers' teaching. An English-Chinese index is attached at the back of the book for easy inquiry. The book can be used as a textbook for undergraduates and graduate students majoring in nutrition and food. Introduction to catalogue

Chapter 1 the concept of gene expression and the basic theory of gene expression regulation

Section 1 the basic concept of gene expression

Section 2 the basic theory of gene expression regulation

Section 3 the significance of gene expression regulation

Chapter II the Regulation of nutrients on Gene expression and their effects on Genome structure and Stability

Section 1 Regulation of gene expression by nutrients

Section 2 effects of nutrients on the structure and stability of genomes

Chapter III Regulation of Gene expression by various nutrients

Section 1 Regulation of gene expression by protein

Section 2 Regulation of Gene expression by Fat

Section 3 Regulation of Gene expression by carbohydrates

Section 4 regulation of gene expression by calcium

Section 5 Regulation of gene expression by iron

Section 6 Regulation of Gene expression by Zinc

Section 7 Regulation of gene expression by selenium

Section 8 Regulation of Gene expression by Chromium

Section 9 Regulation of Gene expression by Vitamin A

Section 10 Regulation of Gene expression by Vitamin D

Section 11 Regulation of Gene expression by Vitamin E

Section 12 regulation of gene expression by biotin

Chapter IV effects of Gene Polymorphism on absorption, Metabolism and Utilization of nutrients

Effect of Gene Polymorphism on folic Acid Metabolism

Section 2 the effect of vitamin D receptor gene polymorphism on calcium metabolism

Section 3 effect of Gene Polymorphism on cholesterol Metabolism

Section 4 effect of gene polymorphism on iron metabolism

Section 5 Gene Polymorphism and lactose intolerance

Chapter V the role of interaction between nutrients and genes in the Pathogenesis of Diseases

Section 1 Overview

Section 2 effects of early life nutrition on obesity-related diseases in adults

Section 3 effect of interaction between Nutrition and Gene on Hyperlipidemia

Section 4 the effect of interaction between nutrients and genes on the occurrence of Obesity

Section 5 effects of interaction between nutrients and genes on salt-sensitive hypertension

Section 6 effect of interaction between nutrients and genes on the occurrence of Diabetes Mellitus

reference

Attachment: you need to log in to download or view the attachment. No account number? Registration-2. Molecular Nutrition author: (American) J. Zeng Purney, (de) H. Edited by Daniel, translated by Luo Xugang, etc.

Publishing house: science Press

Publication time: 2008-5-1 words: 614000 editions: 1 pages: 414 Printing time: 2008-05-01 opening: 16 openings: 1 Paper: offset Paper I S B N: 9787030173713 content introduction

Molecular nutrition is the fastest growing science in the field of nutrition. This book is the only Chinese translation of research progress in the field of molecular nutrition published in China. The latest and most important research results on molecular nutrition published by international top experts are reviewed in the book. The book is divided into 24 chapters. The research methods of molecular nutrition, the dynamic balance of intracellular nutrients, cell proliferation and apoptosis, signal transduction, gene expression and proteolysis, the physiological effects of nucleic acid and molecular events, and the molecular mechanisms of genetically modified food and food allergy are described in detail.

This book is faithful to the original text and reflects the style and charm of the original book to the maximum extent. It can be used as a reference book for teaching, researchers and graduate students majoring in nutrition, animal nutrition, biochemistry, molecular biology and medicine. Catalogue

Translator's preface

Preface

Part 1: methods of molecular nutrition research

1 Genome and post-genome

Ji Zhang

2 the prospect of post-genomic nutrition research

Hannelore Daniel

Part 2: cellular nutritional balance, cell proliferation and apoptosis

3 Molecular physiology of organic nutrient plasma membrane transporters

Hannelore Daniel

Intracellular transport and compartmentalization of vitamins and their physiologically active forms

Donald B.McCormick

5 nutrient balance in proliferating cells

Janos Zempleni

6 Nutrition and apoptosis

John C.Mathers

Part 3: the role of nutrients in signal transduction, gene expression and proteolysis

7 regulation of gene expression by glucose in mammals

Fabienne Foufelle and Pascal Ferre

Amino acid-dependent transcriptional regulation in 8 NgL animal cells

Van Leung-Pineda and Chin Chen of Michael S. Kilberg

9 fatty acids and gene expression

Ulrike Beisiegel,Joerg Heeren and Frank Schnieders

The role of retinoic acid receptor and retinoic acid X receptor in regulating the molecular mechanism of vitamin An action

Dianne R.Soprano and Kenneth J.Soprano

Regulation of gene expression by biotin, vitamin B6 and vitamin C

Krishnamurti Dakshinamurti

12 selenium and vitamin E

Alexandra Fischer,Josef Pallauf,Jonathan Maj ewicz,Anne Marie Minihane and Gerald Rimbach

13 sphingolipids: a new strategy for cancer treatment and prevention

Eva M.Schmelz

14 the health function of isoflavones in food

Thomas M. Badgerjer Martin J.J.Ronis and Nianbai Fang

15 ubiquitous proteolysis and proteasome-dependent proteolysis in skeletal muscle

Didier AttaiX,Lydie Combarct,Anthony J.Kee and Daniel Taulandier

Part 4: nucleic acid and nucleic acid binding compounds

16 Diet, DNA methylation and cancer

Judith K.Christman

17 biotinylation of histones in human cells

Janos Zempleni

18 nicotinic acid nutritional status, metabolism of polymers (ADP- ribose) and genomic instability

Jennifer C.Spronck and James B.Kirkland

Part 5: effects of molecular activities on physiology

19 assembly of plasma lipoproteins transporting triglycerides

Joan A.Higgins

Regulation of cholesterol in 20 cells

Ji-Young Lee,Susan H.Mitmesser and Timothy P.Carr

21 2002: assessment of the effect of nutrition on the incidence of cataract

Allen Tavlor and Mark Siegal

22 Nutrition and immune function

Parveen Yaqoob and Philip C.Calder

Part 6 Food

23 the molecular mechanism of food allergy

J.Steven Stanley and Gary A.Bannon

Safety evaluation of genetically modified food

Steve L.Taylor

Index book excerpt illustration

1 Genome and post-genomic Ji Zhang

(1 Department of Pathology and Microbiology, University of Nebraska Medical Center; 2 Murray Meyer Institute Human Molecular Genetics Center, University of Nebraska Medical Center, Omaha, Nebraska, USA)

Introduction

In recent years, great progress in the field of genome research has promoted the accurate determination of the molecular mechanism of human health and disease, which provides great potential for improving health, reducing morbidity and mortality and preventing diseases. Understanding the molecular mechanisms of different responses of healthy adults to nutritional intake will greatly promote the development of nutrition.Therefore, it is important for nutritionists to acquire knowledge of the necessary technologies and resources for genome research. At first, genomics referred to the scientific rules for mapping, sequencing, and analyzing the genome of an organism, that is, a complete set of genes and chromosomes. At present, the research focus of genomics has shifted from structural analysis (structural genomics) to functional analysis (functional genomics). The purpose of structural genomics is to construct high-resolution genetic, physical and transcriptional maps of organisms, and finally determine all their DNA sequences. However, functional genomics represents a new stage of genome research, which refers to the development of innovative technologies on the basis of a large amount of structural genomics information. The first part of this chapter will focus on the tools and reagents used in structural genomics; the second part will focus on DNA microarray technology, which is the representative of today's functional genomics.

Structural genomics

Genome, genetic Mapping and physical Mapping

The human genome contains nearly 3 billion nucleotide base pairs and carries the genetic code of 30, 000 to 100, 000 genes. The diploid genomic DNA consists of 22 pairs of autosomes and two sex chromosomes. Each chromosome contains a linear DNA molecule, which is characterized by three functional elements that are necessary for successful chromosome replication during cell division: ① self-replicating sequence, ② centromere, the junction of spindle during mitosis or meiosis, and ③ telomere, which ensures complete replication of terminal chromatids. Genome map is defined as the relative position of different loci (genes, regulatory sequences, polymorphic marker sequences, etc.) in DNA molecules. There are two different methods for mapping genomic loci: genetic mapping (genetic mapping) and physical mapping (physical mapping).

……

Attachments: you need to log in to download or view attachments. No account number? Registration-3. Author of Integrated Applied Physiology: editor-in-Chief Feng Zhiqiang

Published by the people's military Medical Publishing House

Publication time: 2006-5-1 words: 540000 editions: 1 page: 345.Printing time: 2006-05-01 edition: printing times: sheet: offset paper I S B N: 9787509102237 content introduction

Under the guidance of materialist dialectics, this book integrates physiological knowledge, related research progress and relevant humanistic knowledge to form a unique knowledge framework. The book consists of 55 chapters, including the development of physiology, the basic characteristics of life, the mechanism of homeostasis, the function of cells, the functional activities and coordination mechanisms of various systems of the body, and the protection mechanism of the body. each section expounds a relatively complex physiological problem in the form of integration, with both breadth and corresponding depth; at the same time, it also pays attention to combining the basic theory of physiology with some clinical changes or research progress. Under the premise of taking physiological knowledge as the core, putting forward new hypotheses or unclarified questions, especially the introduction of the thoughts and spirits of some winners of the Nobel Prize in physiology or medicine, can enlighten readers. This book reorganizes the physiological knowledge with a novel idea, with comprehensive and substantial content, strong integration of graphics and mainly designed for the author. This book is mainly used for graduate students in medical colleges and universities and undergraduates of various majors to learn physiological knowledge and train their thinking on complex problems. It also has reference value for teachers engaged in basic and clinical teaching. Catalogue

Chapter 1 Development of Physiology

Section 1 the development of physiology and the role of materialist dialectics in it

I. understanding of human physiology in ancient times

II. The development of modern physiology

III. The development of modern physiology

IV. The role of materialist dialectics in the development of physiology.

V. thoughts that should be adhered to and problems that should be paid attention to in physiological research

Section II the Development of Physiology in China

I. the development of physiology in China

Second, China's contribution to the development of physiology

III. The contribution of the older generation of physiologists to the development of physiology in China

IV. The development of physiology in China is closely linked with the fate of the motherland.

Section III Macro-level and Micro-level of physiological Research

I. the content of macro and micro level research.

II. Progress and achievements of research at the macro and micro levels

III. The complementary role of macro-and micro-level research.

Section 4 methods and techniques of physiological research

I. methods of physiological research

Intracellular recording technique and extracellular recording technique

III. The development of physiological research conditions.

Chapter 2 basic characteristics of Life

Section 1 Metabolism

I. Metabolism and its two aspects

Second, the relationship between metabolism and the function of the whole system.

Section 2 excitability and its manifestation

I. the concept of excitability

Second, the expression form of excitability

Section 3 occurrence and Movement of Life

I. the occurrence of life

II. Individual growth and development and its regulation

III. The meaning of life and its attitude towards life

Section IV Information Transmission in Life activities

1. Major events in the study of cellular information transmission

Information substances in the body and their movements

Third, the function and mechanism of information transmembrane transmission.

IV. Information transmission and characteristics in life activities

Section 5 Coordination and mechanism between the body and the environment

Chapter 3 steady-state mechanism

Section 1 Regulation and automatic control of functional activities of the body

Section 2 Physicochemical properties and stability mechanism of intracellular and extracellular fluid

Section 3 Acid-base balance in body fluids and its maintenance Mechanism

Section 4 stable mechanism of plasma osmotic pressure and changes of humoral osmotic pressure

Section 5 the stabilization mechanism of blood pressure

Section 6 absorption and excretion function and its role in homeostasis

Section 7 the stabilization mechanism of body temperature

Chapter 4 the function of cells

Section 1 active and passive transport function of cell membrane

Section 2 Ion channels and membrane currents on the cell membrane

The changes and mechanism of nerve cell activity in section 3

The process and mechanism of synaptic transmission

Motor function of muscle cells in section 5

Section 6 function, production and destruction of red blood cells

Section 7 Electrical activity and physiological characteristics of myocardial autonomic cells and non-autonomic cells

The role of ions in the activity of ventricular myocytes

Chapter 5 functional activities and coordination mechanisms of various systems of the body

The first section of the heart's pumping function and its changes

Section 2 the formation mechanism of arterial blood pressure

Section 3 functional activities and regulation of microcirculation

Section IV Regulation of Cardiovascular activities

Section 5 formation and regulation of respiratory movement

Section 6 Exchange and Transportation of gases

Section 7 Digestive function and its Coordination

Section 8 the mechanism of hunger and satiety

Section 9 Movement and regulation of energy substances in the body

Section 10 the process of urine production and excretion and its regulation

Section 11 Regulation of autonomic functional activities of the body

Section 12 the process and mechanism of visual production

Section 13 the process and mechanism of hearing production

Section 14 pain and analgesia

Section 15 other sensory functions of the body

Section 16 changes and regulation of body functions and activities during exercise

Section 17 stretch reflex and reverse stretch reflex

Section 18 maintenance mechanism of body posture and movement

Section 19 Awakening and Sleep

Section 20 memory and forgetting

Section 21 unconditioned and conditioned reflexes

Section 22 regulation of the cerebral cortex on the functional activity of the body

Section 23 function and regulation of endocrine and exocrine

Section 24 the effect and mechanism of hormone on the regulation of receptor

Section 25 Synergistic and antagonistic effects of hormones

Section 26 the role of sex hormones and the regulation of secretion

Chapter 6 Protection Mechanism of the body

Section 1 immune function of the body

Section 2 Blood Coagulation and Anticoagulant Mechanism

Section III Intragastric injury factors and gastric self-protection

Section 4 the response and mechanism of the body to emergency

Section 5 damage factors in the environment and the protection mechanism of the body

reference

Attachments: you need to log in to download or view attachments. No account number? Registration-these books are good, especially the third book Integrated Physiology, which is very good for a comprehensive understanding of animal bodies! -- 4. Molecular biology and genomics author: (de) Muhad edited by

Publishing house: science Press

Publication time: 2007-7-1 words: 421000 editions: 1 page: 257Printing time: 2007-07-01 opening: printing: paper sheet: offset Paper I S B N: 9787030193896 package: hardcover Classification: books > > Natural Science > > Bioscience > Genetics

Content introduction

At present, one of the major puzzles faced by laboratory staff is how to use the right tools and methods to explain the structure, function and expression of the related proteins encoded by the genome. Molecular Biology and Genomics by Muelhardt of the University of Marburg in Germany provides many useful laboratory tips and techniques, which can greatly improve the success rate and accuracy of the experiment. This laboratory guide briefly introduces various modern and conventional methods involved in molecular biology and genomics, and discusses the advantages and disadvantages of each method.

As one of the "experimenter" series, this book adheres to the consistent style of the series, and is still a very practical laboratory manual with more than 100 charts in a simple and lively style. for the majority of molecular biology and genomics laboratory staff to provide a large number of practical tips to supplement and improve the basic knowledge, so as to greatly improve the accuracy and success rate of the experiment.

This book is suitable for laboratory staff, researchers, teachers, senior undergraduates and graduate students in the field of molecular biology and genomics. Catalogue

Introduction

Preface to the first edition

Thank you

1 what is molecular biology?

1.1 basic knowledge of molecular biology: let beginners understand the molecular world

1.2 basic requirements of molecular biology

1.3 Laboratory safety

2 introduction of basic methods

2.1 differences in nucleic acids

2.2 Nucleic acid precipitation and concentration

2.2.1 ethanol precipitation method

2.2.2 concentration plant

2.2.3 freeze drying

2.2.4 salting-out

2.3 Nucleic acid purification

2.3.1 extraction and purification of phenol and chloroform

2.3.2 Polyethylene glycol precipitation method

2.3.3 purification by protein binding filter membrane

2.3.4 purification by anion exchange column

2.3.5 purification of glass emulsion

2.3.6 cesium chloride density gradient centrifugation

2.3.7 dialysis method

2.4 determination of nucleic acid concentration

2.4.1 Spectrophotometer method

2.4.2 agarose gel electrophoresis

2.4.3 Point quantization method

2.4.4 fluorescence quantitative method

2.4.5 nucleic acid detection method

2.4.6 enzyme labeling method

2.5 DNA preparation method

2.5.1 small-scale preparation of plasmid DNA

2.5.2 Mass preparation of plasmid DNA

2.5.3 bacterial medium

2.5.4 preparation of bacteriophage DNA

2.5.5 preparation of single-stranded DNA using helper phage

2.5.6 preparation of genomic DNA

3. Molecular biology tools

3.1 restriction enzymes

3.1.1 Nomenclature

3.1.2 activity determination

3.1.3 restriction enzyme digestion

3.1.4 difficulties in restriction enzyme digestion

3.1.5 guidelines for restriction enzyme digestion

3.2 Gel

3.2.1 agarose gel

3.2.2 recovery of DNA fragments from agarose gel

3.2.3 Polyacrylamide gel

3.2.4 recovery of DNA fragments from Polyacrylamide Gel

3.2.5 Pulsed field gel electrophoresis

3.2.6 Capillary Electrophoresis

3.3 imprinting method

3.3.1 Sotlthern blotting

3.3.2 Northern blotting

3.3.3 Dot imprinting and seam imprinting

4 polymerase chain reaction

4.1 Standard polymerase chain reaction

4.2 improved polymerase chain reaction

4.2.1 nested polymerase chain reaction

4.2.2 Multiplex polymerase chain reaction

4.2.3 long fragment polymerase chain reaction

4.3 Application of polymerase chain reaction

4.3.1 reverse transcription polymerase chain reaction

4.3.2 Rapid Amplification of cDNA ends

4.3.3 amplification of the same product

4.3.4 Classical quantitative polymerase chain reaction

4.3.5 Real-time quantitative polymerase chain reaction

4.3.6 reverse polymerase chain reaction

4.3.7 Biotin-RAGE polymerase chain reaction

4.3.8 modified primer mutagenesis

4.3.9 Amplification retardation mutation system

4.3.10 in situ polymerase chain reaction

4.3.11 cyclic sequencing

4.3.12 cDNA synthesis

4.3.13 single cell polymerase chain reaction

5 RNA

5.1 inactivation of RNA enzyme

5.2 RNA extraction method

5.2.1 one-step method

5.2.2 Ethylphenyl polyethylene glycol lytic method

5.2.3 basic Information

5.2.4 determination of RNA concentration

5.3 mRNA separation method

5.3.1 purchase RNA

5.4 reverse transcription: cDNA synthesis

5.5Transcription in vitro: RNA synthesis

5.6 RNA interference

6 Clone DNA fragment

6.1 basic elements of cloning

6.1.1 Carrier

6.1.2 DNA fragments

6.1.3 toning reaction

6.1.4 quantitative DNA

6.1.5 connection

6.1.6 cloning PCR products

6.1.7 cloning with recombinant enzyme system

6.2 Select cloning vector

6.2.1 plasmid

6.2.2 bacteriophage

6.2.3 Coase plasmid

6.2.4 P1 artificial chromosome and bacterial artificial chromosome

6.2.5 yeast artificial chromosome

6.3 Select bacteria

6.4 preparation of competent cells and transformation

6.4.1 calcium chloride method

6.4.2 Rubidium chloride method

6.4.3 TSS method

6.4.4 Electric transfer

6.4.5 titer test

6.5 issues related to cloning

6.6 Preservation of clones

7 Hybridization: how to detect DNA

7.1 preparation of probe

7.1.1 method of preparing labeled probe

7.2 Hybridization

7.2.1 Hybridization buffer

7.2.2 Hybridization reaction system

7.2.3 Hybridization temperature

7.2.4 washing

7.3.Verification of tagged DNA

7.3.1 autoradiography

7.3.2 non-radioactive detection method

7.4 screening of recombinant DNA library

7.4.1 preparation of DNA library plate

7.4.2 transfer of membrane

7.4.3 Hybridization of membrane

7.4.4 exposure of film

7.4.5 Clone probe

7.4.6 Future screening of libraries

7.5 two-hybrid system

8 DNA analysis

8.1 sequencing

8.1.1 Radiological sequencing

8.1.2 non-radioactive sequencing and automatic sequencing units

8.1.3 Pocket sequencing

8.1.4 pyrophosphate sequencing method

8.1.5 sequencing characteristics: octamers

8.2 methods for analyzing DNA mutations

8.2.1 restriction fragment length polymorphism

8.2.2 single strand conformation polymorphism

8.2.3 denaturing gradient gel electrophoresis

8.2.4 Phase temperature gradient electrophoresis

8.2.5 heteroduplex nucleic acid analysis

8.2.6 Amplification blocking mutation system

8.2.7 mismatched enzyme digestion

8.2.8 truncated protein detection

Study on the function of 9 DNA sequence

9.1 study on intra-tissue transcription

9.1.1 Ribonuclease protection test

9.1.2 Real-time quantitative PCR experiment

9.1.3 in situ hybridization

9.1.4 chromosome fluorescence in situ hybridization

9.1.5 in situ polymerase chain reaction

9.1.6 Microarray

9.2 mutation

9.3 in vitro translation

9.4 expression system

9.4.1 bacterial expression system

9.4.2 baculovirus expression system

9.4.3 other expression systems

9.4.4 heterologous expression system in mammalian cells

9.4.5 transfection method

9.4.6 Co-transfection of multiple genes

9.4.7 transient and stable transfection

9.4.8 reporter gene

9.5 transgenic mice

9.5.1 transgenic methods

9.6 Regulation of transgene expression

9.6.1 tetracycline gene expression regulation system

9.6.2 Regulation system of ecdysone gene expression

9.7 gene therapy

9.8 Genomics

10 computer applications

10.1 important matters

10.2 problems in practice

11 career planning advice: Machiavelli short course for young researchers

12 concluding remarks

Appendix 1

Icon

Standard solution and bacterial medium

Solution

Bacterial culture medium

Glossary of terms

Appendix 2

Supplier

Recommended literature

Leisure reading material

Indexes

Attachments: you need to log in to download or view attachments. No account number? Registration-there is no electronic version?.-- 5. Protein Biochemistry and Proteomics Click to view the larger picture by (de) Rem

Publishing house: science Press

Publication time: 2007-1-1 words: 350000 editions: 1 page: 236. printing time: 2007-01-01 edition: printing: paper sheet: offset paper I S B N: 9787030182180 package: hardcover category: books > > Natural Science > > Bioscience > > Biophysics

Customer rating: a total of 2 customer comments to view the summary of the review summary

In the experiment, did you strictly follow the standard procedure and get nothing? Do you want to improve your work? Do you want to know more about the various methods in a particular field of research? Maybe the "experimenter" series will help you.

This book is part of the "experimenter" series, which tells you about new methods of protein biochemistry and proteomics from the point of view of pundits. You can fully appreciate the excitement and frustration brought to them by the author who has tasted the challenges of the laboratory. With these skills and tricks, your experiment will have a higher chance of success. But this valuable laboratory manual is not a human being, but a collection of methods:

It shows you the way out of the predicament of the experiment and cultivates your intuition to choose the right experiment at the right time.

It succinctly outlines the steps of conventional methods (such as column chromatography, gel electrophoresis) and lists the advantages and disadvantages of different methods.

It introduces in detail the progress in ligand binding experiment, antibody production and microsequence analysis.

It deals with some special methods, such as the dissolution and recombination of membrane proteins, the analysis of glycoproteins, or the determination of oligomeric protein subunits.

One section gives a directional explanation of proteomics.

Not only that, but like other books in the system, this book guides you out of the Night of the Soul with inspiring honesty when you doubt yourself and don't know why you're doing it.

This book is suitable for research institutes in life science related fields such as biochemistry and molecular biology, genetics, functional genomics, protein biochemistry and proteomics, as well as teachers, graduate students and researchers in laboratories related to colleges and universities. as well as biotechnology enterprise developers and decision-makers for reference. Catalogue

Preface

Preface to the fourth edition

Thank you

Acronym

1 conventional technology

1.1 preparation of buffer solution

1.2 protein quantification

1.3 Gel electrophoresis

1.4 Gel staining

1.5 precipitation and enrichment

1.6 imprinting

1.7 autoradiography of gels and imprints

2 ligand binding

2.1 radioactive ligand labeling

2.2 combination

2.3 Analysis of combined data

2.4 Ligand cross-linking

2.5 use of binding proteins

3 dissolution of membrane proteins

3.1 detergent

3.2 dissolve

(4) protein was detected by functional determination.

4.1 Transporter

4.2 Recombinant reduction of membrane proteins

4.3 Flux experiment

4.4 some constructive comments

5 removal of impurities and purification

5.1 Pure entertainment-the process of protein purification

5.2 conventional purification methods

5.3 Affinity Chromatography

5.4 Purity test

5.5 the effect of purified protein

6 antibody

6.1 acquisition of polyclonal antibodies

6.2 Immunoprecipitation

6.3 Immunoaffinity chromatography

6.4 Antibodies against impure proteins

6.5 immunological detection techniques

7 Proteomics

7.1 introduction

7.2 sampling

7.3 two-dimensional gel electrophoresis

7.4 Peptide and protein Mass Spectrometry

7.5 protein chip

7.6 microsequence analysis

7.7 Strategic perspective

8 subunit

The number and proportion of 8.1 subunits

8.2 what forces keep us together? -- binding between subunits

9 glycoprotein

9.1 the way, site and function of protein glycosylation

……

10 cohesion Treasure: the Writing of papers

11 Desert Exploration: a Survey of Literature

Appendix A Professional Resources

Indexes

Attachments: you need to log in to download or view attachments. No account number? Registration-the original post was posted by Ronnie Rosso at 11:19 on 2008-6-27, without an electronic version. I'm sorry I don't-- I've learned a lot! What an eye-opener! -6. Click on immunology to view the large picture by (de) Ruitman (Luttmann,W.) et al.

Publishing house: science Press

Publication time: 2007-1-1 words: 409000 editions: 1 page: 243Printing time: 2007-01-01 edition: printing times: paper sheet: offset paper I S B N: 9787030182197 package: hardcover category: books > > medicine > > basic medicine

There are 0 customer comments to view the summary of the comments.

This book is one of the "experimenters" series. The book is divided into ten chapters, which comprehensively discusses antibodies, the sources of antibodies, their effects in the whole, and their important role as an immunological tool. This book introduces flow cytometry, immunoblotting, ELISA and related immunological tests, up to cell separation and in situ immunolocalization, and points out some problems that should be paid attention to in biostatistics. Like other books in the experimenter series, this book focuses on explaining modern methods in an easy-to-understand way and discussing the advantages and disadvantages of each.

Due to the rapid development of immunology and related disciplines, although the experimental methods introduced in this book are not all up-to-date and comprehensive in all fields of immunology, the methods introduced in this book are the most practical and reliable. It not only describes the principle of the method, but also introduces the experimental steps in detail, analyzes the reasons for the success or failure of the experiment, and compares it with its local methods. In particular, the "tips" are used to remind the experimenters to pay attention to the problems that may arise in the experiment, how to solve them, how to protect the safety of the operators and so on. This book also provides some useful charts, background information, glossary, modern CD naming table, and related commodity information to help immunology work in the laboratory.

The book will be of great reference value for students, teachers and researchers engaged in basic medicine, clinical medicine and biology related to immunology. Catalogue

Preface

Appendix

Acronym

1 antibody

1.1 characteristics of antibodies

1.2 preparation of antibodies

1.3 Antibody purification

1.4 Chemical cross-linking and labeling of antibodies

2 cell isolation

2.1 Separation according to cell size and density: centrifuge technique

2.2 by the separation of specific molecules on the cell surface

3 flow cytometry

3.1 how it works

3.2 fluorescent substances

3.3 sample preparation

3.4 initiation of flow cytometry ritual

3.5 compensation and measurement

3.6 Analysis

3.7 Mode and configuration

4 quantitative immunity test

4.1 Test principle

4.2 radioimmunoassay (RIA)

4.3 enzyme linked immunosorbent assay (ELISA)

4.4 enzyme-linked dot test (ELISPOT Assay)

4.5 microparticle immunity test (PIA)

4.6 magnification series

5 immunoblotting

5.1 sample preparation

5.2 protein separation by gel electrophoresis

5.3 protein transferred south to the membrane (imprinting)

5.4 protein determination

5.5 spot and slit imprinting

6 in situ immunolocalization

7 immunoprecipitation

8 cell survival, phagocytosis and death

9 special immunological tests

10 Experimental error statistics

Appendix CD antigen

Terminology (professional glossary)

Indexes

Attachments: you need to log in to download or view attachments. No account number? Registration-the prices of these books are not cheap, and it is against the copyright law to send electronic versions here. Besides, molecular biology is only one of the means of nutrition research, and the principles are all common. Experimental Manual of Cell Biology (third Edition) Volume 1 by (Dan) Selis (Celis,J.) Editor-in-Chief

Publishing house: science Press

Publication time: 2008-1-1 words: 1115000 editions: 1 page: 586Printing time: 2008-01-01 opening: 16 openings: 1 sheet: offset Paper I S B N: 9787030203830 package: hardcover Classification: books > > Natural Science > > Bioscience > > Cytology

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Like the second edition of this book, this edition is divided into four volumes. The first volume includes tissue culture and its related techniques, viruses, antibodies and immunohistochemistry. The second volume covers organelles and cellular structures, as well as cell biological detection techniques. Volume III deals with imaging techniques, electron microscopes, scanning probes and scanning electron microscopes, microanatomy, tissue matrix, cytogenetics and in situ hybridization, genomics, transgenes, gene knockout and gene deletion methods. The last volume includes macromolecular transfer, expression systems, and gene expression models in addition to various proteomic techniques. The appendix collects the representative cultured cell lines and their characteristics, cell biology Internet resources, and bioinformation resources in the computer simulated proteome analysis system. This manual can uniquely provide classical and up-to-date technologies that are indispensable for research in life sciences. If you are short of experts, this manual can help you use a variety of technologies and model systems to study biological problems at any stage of your scientific research career. The techniques introduced in this book are described in a humane, step-by-step manner, and teach you some useful tips to avoid the small troubles you may encounter in the experiment.

This book features:

Indispensable tool books and experimental partners in biology laboratory

Track cutting-edge technologies and provide up-to-date information to solve complex biological problems

Use a large number of illustrations, some of which are full-color pictures to demonstrate the operation steps and results.

Guide version:

The preface and catalogue of this book have been translated into Chinese, and the original text is retained in English, with a Chinese guide written by Professor Zhang Jingbo, doctoral supervisor of the Institute of basic Medicine, China Union Medical University, Chinese Academy of Medical Sciences. Content introduction

This manual covers the classical experimental schemes of cell biology and has important guiding significance for life science research. The updated third edition includes 165 new articles, covering classics and the latest experimental techniques. Various technologies are gradually carried out in the form of humanization, and some practical skills and mistakes are introduced. Important experimental steps and results are illustrated with illustrations, which are easy to understand and use. With the collation and publication of this book, researchers at different levels can use corresponding technologies and system models to solve basic biological problems in the process of scientific research.

Volume 1: techniques related to cell and tissue culture, viruses, antibodies, immunocytochemistry.

Volume 2: organelles and cellular structure, as well as cell biological detection techniques.

Volume 3: imaging, Microscopy, tissue Matrix, Cytogenetics and in situ Hybridization, genetic Engineering and Genomics.

Volume 4: macromolecular transfer, expression system, gene expression model, protein. Catalogue

Volume 1 catalogue

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Contributor

Preface

A cell and tissue culture: related techniques

Part 1 General Technology

1. Establishment of cell culture laboratory

two。 General procedures for cell culture

3. Cell count

4.

5. Research and development of serum-free medium: optimization of nutrients and delivery mode

6. Cell line identification

7. Detection of cell line microorganisms and virus contamination

Article 2 Culture of special cell types: stem cells

8. Neural crest stem cells

9. Bone Stem cells from Neonatal Animals: a method for isolation and Analysis of Bone Marrow Stromal cells BMSCs from Neonatal mice and Human Bone Marrow

10.

11. Isolation and proliferation of T cells in vitro

twelve。 Generation of human and mouse dendritic cells

Article 3 Culture of special cell types: hematopoietic cells, mesenchymal cells and epithelial cells

13. Basic Clone Culture of Hematopoietic cells in semi-solid Agar in Vitro

14. Human skeletal muscle cells

15.

16. Culture of human epidermal keratinocytes and retrovirus infection

17. Three-dimensional culture of normal and malignant human breast epithelial cells to maintain architecture such as in vivo

18. Primary culture of Drosophila embryonic cells

19. Laboratory culture of nematodes and other non-symbiotic nematodes

Chapter 4 differentiation and reprogramming of somatic cells

Chapter 5 Immortality

Article 6 somatic hybridization

Chapter 7 Cell isolation techniques

Article 8 Cell cycle analysis

Article 9 determination of cytotoxicity and cell growth

Article 10 apoptosis

Article 12 Electrophysiological methods

Chapter 13 Organ Culture

Chapter 14 growth and purification of viruses

C antibody

Part 15 production and purification of Antibodies

D immunocytochemistry

Article 16 immunofluorescence

E Appendix

Preface to Volume II

An organelle and cellular structure

Part 1 Separation: plasma membrane, organelle, cell structure

1 detergent resistant film and cholesterol removal

2 separation and re-classification of plasma membrane and purification of cell fossa from lipid raft

3Immunoseparation of organelles with magnetic solid phase support

4 isolation of Golgi complex in rat liver

5 isolation of the domains of rough and smooth membranes of endoplasmic reticulum in rat liver

Purification of 6 COPI vesicles

7 purified grid protein coated vesicles from midbrain, liver and adrenal gland

9 separation of peroxides

11 subcellular fractionation and isolation of dense core secretory granules from neuroendocrine cell lines by [S] salt metabolic markers

12 preparation of synaptic vesicles from mammalian brain

Preparation of proteasome 13

14 preparation of cilia from human tracheal epithelial cells

Separation of 15 nucleoli

16 purification of splicing bodies containing intermediates

Isolation of 17 Cajal bodies

18 replication clusters: marker strategies for chromosome construction and chromatin kinetic analysis

Chromosome 19 segregation for flow analysis and sorting

Chapter 2 in vivo staining of cells / organelles

20 in vivo staining of cells with fluorescent lipids

Part 3 protein purification

Preparation of 22 porcine brain tubulin

Purification of 23 flat muscle actin

……

B determination method

Article 4 endocytosis and exocytosis pathway

Chapter 5 membrane

Article 6 Pure material body

Chapter 7 Nuclear Transport

Article 8 chromatin assembly

Chapter 9 signal transduction test

Chapter 11 Mechanical stress in single cells

Appendix C Volume III

Preface

An imaging technology

Part 1 Optical Microscopy

1. Fluorescence microscopy

two。 Total internal reflection fluorescence microscopy

3. Frequency limitation and proper sampling in microscopy

4. Optical tweezers: application in the study of dynamic protein

Part 2 Digital Image Microscopy

5. How to obtain Electronic Images in Optical microscope observation of Biological specimens-- introduction

6. Image enhancement contrast microscopy

Article 3 Confocal microscopy of living cells and fixed cells

7. Confocal microscopy of living cells by rotating disc

8. Confocal microscopy of Drosophila embryos

9. Application of Ultraviolet Laser microbeam in Drosophila embryo Anatomy

Part 4 fluorescence microscopy of living cells

10. Brief introduction of fluorescence imaging of living cells: itemized notes

11. Cytoskeleton protein

twelve。 Systematic cellular localization of new proteins

13. Imaging of single molecule of living cells by total internal reflection fluorescence microscope

14. Live cell fluorescence dot microscopy for actin cytoskeleton kinetics and drug perfusion interference

15. Transfer of fluorescence resonance energy between green fluorescent protein variants in living cells

Part 5 study of intracellular physical parameters using fluorescent dyes

16. Determination of pH value of endocytosis in living cells by double excitation fluorescence imaging

17. Genomic range screening of intracellular protein location in Schizosaccharomyces cerevisiae

18. Large-scale protein localization in yeast

Part 6 Digital Imaging production, Analysis, Storage and demonstration

19.

20. Overview of techniques in Biological Imaging Analysis

21. Imaging data in biological imaging database, an imaging database of biologists

B electron microscope

Part 7 preparation techniques of specimens

twenty-two。 Fixation and embedding of cells and tissues in Transmission Electron Microscopy

23. Negative dyeing technology

24. Glycerol spray / low-angle rotating metal projection

Article 8 constant cooling technology

25.

twenty-six。 Freeze fracture and freeze etching

Chapter 9 Electron microscopic study of cytoskeleton

twenty-seven。 Electron microscopy for cytoskeleton extraction: negative staining, cryogenic electron microscopy, and its correlation with optical microscopy

twenty-eight。 The relationship between cytoskeleton optics and electron microscopy

Article 10 immune electron microscopy

twenty-nine。 Immune electron microscopy with Lowicryl resin

thirty。 Immunocytochemistry of ultra-thin frozen and plastic sections

thirty-one。 Direct immunogold labeling of protein complex components

C scanning probe and scanning electron microscope

Chapter 11 scanning probe and scanning electron microscope

thirty-two。 Atomic force micromirror in biological research

thirty-three。 Field emission scanning electron microscope and internal observation of cells

D microanatomy

Chapter 12 Microanatomy of tissues and chromosomes

thirty-four。 Laser capture microanatomy

thirty-five。 Microdissection of chromosomes by conventional methods

thirty-six。 Micromanipulation of chromosomes and mitotic spindles with laser microsurgery (laser scissors) and laser generated optical forces (laser forceps)

E organization matrix

Chapter 13 Organization Matrix

thirty-seven。 Tissue micromatrix

F cytogenetics and in situ hybridization

Article 14 Cytogenetics

thirty-eight。 Basic cytogenetic techniques: culture, film production and G-banding

thirty-nine。 A common and reliable method for obtaining high-yield metaphase chromosome samples from attached growth cell lines: rapid confirmation of cell chromosome components

Chapter 15 in situ Hybridization

forty。 Mapping of clonal DNA on metaphase chromosomes by fluorescence in situ hybridization

forty-one。 Using Human Genome Project resources for breakpoint mapping

forty-two。 Fine mapping of gene sequencing by extended staining

forty-three。 It is suitable for interspecific hybridization of mRNA in cultured cells.

forty-four。

forty-five。 Fluorescence observation of Genome structure and DNA replication at single Molecular level

G genomics

Article 16 Genomics

forty-six。 Genomic DNA micromatrix for comparative genomic hybridization

forty-seven。 Genotyping of single nucleotide polymorphisms by tag matrix and microsequence determination

forty-eight。 Analysis of single nucleotide Polymorphisms by Matrix Laser Desorption / Ionization Mass Spectrometry

forty-nine。 Analysis of single nucleotide Polymorphism by ZipCode tagged Microsphere technique

fifty。 PCR Amplification method for maintaining the quantitative difference between two complex genomes

Methods of H transgenic, gene knockout and gene deletion

Chapter 17 methods of transgene, gene knockout and gene deletion

fifty-one。 Generation of transgenic mice by prokaryotic microinjection

fifty-two。 Gene targeting by homologous recombination of embryonic stem cells

fifty-three。 Conditional gene knockout: Cre-loX system

54.RNAi-mediated gene silencing in mammalian cells

fifty-five。 Antisense oligodeoxynucleotides

Volume 4 catalogs other directories

Contributor

Preface

Transfer of macromolecules A

Part 1 protein

Article 2 genes

Article 3 somatic nuclear transfer

B expression system

Chapter 4 expression system

C gene expression model

Article 5 differential gene expression

D protein

Part 6 protein identification and analysis

Part 7. Classification of sample components in proteomics

Chapter 8 Gel Electrophoresis

Article 9 Detection of proteins in Gel

Chapter 10 Gel pattern analysis of post-translational modified proteins

Chapter 11 protein / protein and protein / small molecule interactions

Part 12: functional protein genomics

Chapter 13 protein / DNA interaction

Article 14 protein degradation

Chapter 15 Mass Spectrometry: protein Identification and interaction

E

Appendix 16

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