Dr. Zhou Wenzong's Academic Achievements in Ecological Culture of Monopterus albus and Loach

Dr. Zhou Wenzong is a researcher of Institute of Biotechnology of Nanjing University, a distinguished researcher of Shanghai City to attract domestic talents to work in Shanghai, and a member of Chinese Geographical Society and Chinese Ecological Society. Specialization: ecological engineering and aquatic biology. Research interests: animal ecology and ecological culture (mainly for eel, loach and crayfish), ecological engineering and water restoration. July 1990 Graduated from university and returned home to explore artificial breeding of finless eel. In June 1996, he obtained a master's degree in ecology from Shenyang Agricultural University. Graduated and assigned to Institute of Geography, Henan Province Academy of Sciences to engage in ecological engineering research, and was appointed Director of Ecological Environment Research Center, Henan Academy of Sciences. In 2001, in order to further improve his theoretical level, Zhou Wenzong simultaneously studied for a doctor's degree in ecological engineering from the Academy of Life Sciences of Nanjing University (instructor: Professor Admire of Nanjing University) and a doctor's degree in aquatic biology from the Institute of Aquatic Biology of the Chinese Academy of Sciences (instructor: Academician Liu Jiankang of the Chinese Academy of Sciences). In 2006, he received a double doctor's degree. Zhou Wenzong has successively presided over the completion of 24 projects (projects), successively presided over the provincial scientific and technological key projects "Research on the Development and Application of Ecological Engineering of eel","Comprehensive Research on the Scale Breeding Technology of eel" and "Research on the Integrated Prevention and Control Technology of eel Diseases and Pests", participated in the "Tenth Five-Year Plan" national major scientific and technological projects, the national scientific and technological key projects, 863 plan and 12 provincial projects; He has published more than 80 academic papers at home and abroad, edited 2 books, won the second prize of Science and Technology Progress Award of Ministry of Education in 2009, the second prize of Science and Technology Progress Award of Shandong Province in 2010 and the second prize of Science and Technology Progress Award of Henan Province in 2010 respectively; holds 7 national invention patents as the first inventor, invented and mass-produced "Duoyishan" series drugs and feed additives, and achieved good social and economic benefits. Zhou Wenzong successfully achieved a breakthrough in soilless semi-artificial propagation technology of Monopterus albus in shallow water. He took the lead in carrying out systematic research on earthworm decomposition treatment technology of urban sludge, put forward ecological engineering for treatment and utilization of livestock and poultry waste, initiated "Sanhuang (i.e. yellow cattle, eel and cucumber) economic model", and established demonstration project bases in Xuchang, Zhengzhou, Zhumadian, Nanjing and Fuyang, with a total investment of more than 80 million yuan. I. Zhou Wenzong's Major Academic Innovation in Ecological Breeding 1. A new soilless ecological culture model of ricefield eel in still water was put forward for the first time, and the history of ricefield eel culture by plastic film and successful ricefield eel culture in north China was initiated. The disadvantages of cage culture were overcome.(It cannot be popularized and applied in water shortage areas; the water surface is deep, and the young eel fry are not easy to domesticate; the water surface is large, and fish diseases are easy to infect and not easy to disinfect; the mesh is easy to block and needs to be cleaned frequently, otherwise it will affect the exchange of water inside and outside the box; the feed waste is widespread, the water pollution is serious; the management operation is inconvenient, and the labor intensity is high; the breeding season is concentrated, the breeding time is too short, and the breeding cost is increased; It has the characteristics of low cost, no influence on reclamation, breeding all the year round, saving water and labor, high economic benefit, no pollution and wide adaptation range. The invention patent has been applied for "a method for constructing an ecological eel culture pond"(200910227390.5). 2. A new soilless loach culture technology and net cage culture technology are invented, which overcome the disadvantages of loach pond purse net soilless culture and loach soilless culture at present, and have the characteristics of easy regulation of water quality, feed saving, less loach diseases and insect pests, fast growth speed, improvement of comprehensive benefit of loach culture and the like. The related art has applied for the invention patent "a new soilless loach breeding method (application number: 201010572535.8). 3. A technical route of semi-artificial propagation in shallow water without soil is adopted, namely, parent eel is selected manually, put into a propagation pool in due time, compound feed and live bait are adopted to feed the parent eel in combination, medicines, especially Chinese herbal medicines, are used for accelerating ripening and inducing spawning of the parent eel, natural spawning and fertilization are timely taken out for artificial dripping incubation, and the goal of large-scale propagation is realized. The relationship between reproductive behavior, gonad development and ecological factors of Monopterus albus was explored, and its territory area was found. Monopterus albus without dormancy in winter could also be successfully semi-artificially propagated. 4. Innovatively extract effective components from aquatic plants (especially water peanuts) for drug control, comprehensively utilize resources, adopt ecological technology for integrated pest control, increase biodiversity, fully tap the potential of Chinese herbal medicines, utilize food chain amplification and biological carriers to transfer drugs, improve drug efficacy, and realize environment-friendly breeding. The invention patent "A medicine for preventing and treating aquatic animal diseases and its preparation method"(200410041674.2) has been applied for and the authorized invention patent "Bait and feeding method for wild eel seedlings"(ZL 200410066032.8) has been obtained. 5. A novel eel nest beneficial to eel free entry and exit was invented, which made it possible for eel soilless culture and factory culture. The invention patent of "fish nest and setting method for soilless culture of eel" has been applied (200410066031.3). 6. Epidemiological characteristics of important diseases and insect pests of Monopterus albus in Yangtze River and Yellow River were studied systematically. By soaking eel seeds with the self-developed medicine for several days continuously, eel can be trained with high density, and a series of problems caused by partial shedding of mucus and common tail rot disease of eel in the market can be solved well. The authorized invention patent "Quantitative method for screening wild seedlings of Monopterus albus"(200410066033.2) has been obtained. 7. The feed formula and quality improving additive for different seasons and different growth stages were studied for the first time. The feed coefficient of the developed eel and loach compound feed was low, and a better attractant was found. The technical achievements were transformed by Zhengzhou Saike Biotechnology Co., Ltd., and the products were welcomed by farmers. 8. The feeding ecology and behavior ecology of Monopterus albus were studied systematically. The effects of ecological factors and body weight on the feeding of Monopterus albus were studied systematically, and the maximum feeding rate of Monopterus albus under different body weight and different temperature conditions could be predicted by using a certain equation. The homing behavior of Monopterus albus and the selectivity of Monopterus albus to fish nest under different body weight and water depth were studied. 9. The material and energy flow law of typical artificial food chain was analyzed, which provided theoretical basis for rational design and utilization of food chain (net) of Monopterus albus and reduction of breeding cost. 10. Earthworm ecological filter technology and bioremediation, especially animal remediation technology, were creatively introduced into aquaculture, and a new bioremediation model of microorganism-plant-animal joint remediation was established to achieve the high efficiency goal of eel (loach) symbiosis, bait culture and water purification integration. A patent has been applied for "a method for controlling eutrophication of water bodies by using special food chains"(201010576887.0). 11. For the first time, ricefield eel, loach and Procambarus clarkii were successfully cultured in saline-alkali land. 12. Invented automatic separation technology of earthworm and mealworm. The characteristics of killing each other, the diurnal regularity of pupation and eclosion, and the ability of selenium enrichment were studied. The effects of feed types and feeding density on the growth rate, mortality, pupation rate and nutritional components of Tenebrio molitor larvae were determined. A patent entitled "A new method for harvesting Eisenia foetida"(ZL200410066030.9) has been granted. 13. Take the lead in carrying out systematic research on earthworm decomposition and treatment technology of urban sludge, and establish demonstration project base. The optimum ecological conditions for earthworm decomposition of municipal sludge were studied. The results show that 1 kg earthworm can transform at least 2 kg earthworm dung per day, sludge reduction is more than 30%, heavy metal content is reduced to 3%~44% after earthworm enrichment, and the order of enrichment coefficient of heavy metals by earthworm is Mn>Zn>Ni>As>Cu>Cr>Cd>Hg>Pb. 14. The outbreak of Alternanthera philoxeroides was effectively controlled by using apple snail. It was found that different proportion of water peanut and wheat bran significantly affected the specific growth rate and conversion efficiency of substance and energy of mealworm larvae, and could obviously prolong the development period of mealworm pupae. An equation can be used to predict the maximum feeding rate of apple snails on water peanut under different body weight and temperature conditions. 15. Environmental engineering and ecological engineering technology were integrated in livestock and poultry manure treatment, and anaerobic fermentation technology, biogas slurry breeding loach seed technology, loach and eel symbiosis system, earthworm biological decomposition technology and environmental deodorization technology were integrated to construct a compound ecological engineering and circular economy model for livestock and poultry manure treatment. On November 6, 2008, the project of "Demonstration Research on Ecological Engineering and Circular Economy of Livestock and Poultry Feces Treatment" was appraised by the appraisal committee composed of well-known domestic experts headed by Academician Jin Jianming.

II. Patent Sequence (1)《A Multi-time Intermittent Divider》(Patent No.: ZL 98 2 19385.8);(2) Automatic Calibration Method for δ 15N and δ 13C Values of Biological and Soil Samples (Patent No.: ZL 2003132046.5);(3) A New Method for Harvesting Eisenia fetida (Patent No.: ZL200410066030.9);(4) A Medicine for Preventing and Treating Aquatic Animal Diseases and its Preparation Method (Application No.: 200410041674.2);(5) Quantitative Method for Screening Wild Seedling of Monopterus albus (Patent No.: ZL200410066033.2);(6) Bait and Feeding Method of Wild Rice Field eel Seedling (Patent No.: ZL200410066032.8);(7) Fish Nest for Soilless Culture of Monopterus albus and Setting Method (Application No.: 200410066031.3);(8) A High-density Ecological Transportation Method for Earthworms (Patent No.: ZL20051017239.X);(9) A Method for Prolonging the Pupae Development Period of Tenebrio molitor (Patent No.: ZL 200510107240.2);(10) A Method for Transporting Live Larvae of Tenebrio molitor (Patent No.: ZL 200510107238.5);(11) Method for constructing ecological eel culture pond (Application No.: 200910227390.5);(12) A New Soilless Culture Method for Loach (Application No.: 201010572535.8), unique;(13) A Method for Controlling Eutrophication of Water Body by Using Special Food Chain (Application No.: 201010576887.0).

III. Thesis sequence 1. Zhou Wenzong, Nature Journal, 2003, 25 (4). 2. Key Techniques of Soilless Culture of Monopterus albus Zhou Wenzong, Beijing Aquatic Products, 2003,(4); 3. Zhou Wenzong, Industrialization of Agriculture, 2003,(11); 4. A preliminary study on feeding of live bait by ricefield eel Zhou Wenzong, Li Peiyu. Aquatic Science and Technology Information, 2004, 31 (4); 5. Effect of body weight and temperature on maximum feeding rate of ricefield eel Zhou Wenzong, Li Hongtao, Zhang Luo, Gao Hongli Journal of Hydrobiology, 2008, 32 (6); 6. A Study on the Feeding Rhythm of Protochilus clarkii Zhou Wenzong, Zhao Fenglan, Fisheries Science, 2007, 26 (5); 7. Effect of salinity on feeding rhythm and defecation time of ricefield eel Zhou Wenzong, Mu Zi, Journal of Applied Ecology, 2007, 18 (5); 8. Diurnal Regularity of Pupation and Emergence of Tenebrio molitor Zhou Wenzong, Wang Guang, Journal of Economic Zoology, 2003, 7 (4); 9. Effect of PVC Pipe on Maximum Feeding Rate of Monopterus albus Zhou Wenzong, Li Hongtao, Water Conservancy and Fisheries, 2006, 26 (2); 10. A Preliminary Study on Fecal Activity of Monopterus albus Zhou Wenzong, Zhang Luo, Gao Hongli, Li Hongtao, Acta Hydrobiologica Sinica, 2008, 32 (3); 11. A Study on Material Flow and Energy Flow Conversion Efficiency of Food Chain of Base Feed-Earthworm-eel Zhou Wenzong, Bai Yu, Mu Zi, Chinese Journal of Ecological Agriculture, 2004,(3); 12. Analysis of material flow and energy flow in the food chain of wheat bran-yellow mealworm-eel Zhou Wenzong, Bai Yu, Cai Qinghua, Jiangsu Agricultural Science, 2004,(2); 13. Effect of Salinity on Feeding and Hunger of Monopterus albus Zhou Wenzong, Li Hongtao, Chen Yang, Water Conservancy and Fisheries, 2006, 26 (5) 8; 14. Effect of Spartina alterniflora biominerals on growth and nutritional components of Tenebrio molitor larvae Gao Hongli, Zhou Wenzong, Zhang Luo, Hubei Agricultural Science, 2009, 48 (9); 15. Zhou Wenzong, Zhang Ge, Gao Hongli, Li Hongtao, Journal of Jiangxi Agricultural University, 2007, 29 (1). 16. Law of Monopterus albus preying on several small aquatic animals Zhou Wenzong, Li Hongtao, Gao Hongli, Zhang Luo, Journal of Jiangsu Agriculture, 2007, 23 (2); 17. Diurnal feeding rhythm of ricefield eel with nervous disorder Zhou Wenzong, Zhang Luo, Gao Hongli, Li Hongtao, Journal of Jiangxi Agricultural University, 2007, 29 (2); 18. Effect of PH on maximum feeding rate of Monopterus albus Zhou Wenzong, Li Hongtao, Zhang Ge, Gao Hongli, Jiangsu Agricultural Science, 2007,(1); 19. Analysis of material flow and energy flow in food chain of "Alternanthera philoxeroides and wheat bran-Tenebrio molitor-Monopterus albus" Zhou Wenzong, Mu Zi, Zhang Luo, Gao Hongli, Hubei Agricultural Science, 2007, 46 (1); 20. Effect of Body Weight on Feeding Rhythm of Monopterus albus Zhou Wenzong, Zhang Ge, Gao Hongli, Li Hongtao, Water Conservancy and Fisheries, 2007, 27 (1); 21. Effect of Water Depth on Maximum Feeding Rate and Feed Selectivity of Monopterus albus Zhou Wenzong, Li Hongtao, Zhang Ge, Gao Hongli, Water Conservancy and Fisheries, 2007, 27 (3); 22. Zhou Wenzong, Mu Xi, Zhang Ge, Gao Hongli, Preliminary Study on the Consumption of Water Peanut and Water Lotus by Macdonald Shell and Procambarus clarkii, Hubei Agricultural Science, 2006, 45 (5); 23. Zhou Wenzong, Zhang Luo, Gao Hongli, Li Hongtao, Current Situation and Development Trend of Utilization of Water Peanut Resources in China, Regional Research and Development, 2005, 24 (7); 24. Bioaccumulation of Selenium by Mealworm Larvae Gao Hongli, Zhou Wenzong, Zhang Ge, Li Hongtao, Insect Knowledge, 2007, 44 (6); 25. Test of Combined Toxicity of Salinity and Alkalinity to Loach by Uniform Design Li Hongtao, Zhou Wenzong, Gao Hongli, Zhang Luo, Fisheries Science, 2006, 25 (11); 26. Gao Hongli, Zhou Wenzong, Zhang Luo, Li Hongtao, Effect of Feed Type and Feeding Density on Growth and Development of Tenebrio molitor Larvae, Chinese Journal of Ecology, 2006, 26 (10); 27. Gao Hongli, Zhou Wenzong, Zhang Luo, Li Hongtao, Progress in Earthworm Decomposition Technology of Municipal Sludge, Chinese Journal of Ecological Agriculture, 2008, 16 (3); 28. Papers published in foreign journals (omitted).

IV. Sequence of works Editor-in-chief: Ecological Industry and Industrial Ecology, Ecological Engineering of Special Aquaculture. Associate Editor: Series of Industrial Ecological Engineering, including Biomass Energy Industrial Ecological Engineering, Fermentation Ecological Engineering, Polluted Water Biological Treatment Ecological Engineering, Special Aquaculture Ecological Engineering, Beach Industrial Ecological Engineering, Organic Agricultural Technology and Organic Food Quality, Soil Microbial Ecological Engineering, Special Economic Plant Industrial Ecological Engineering and Textile Industrial Ecological Engineering; Contributed to the preparation of books: "Ecological hot spot research books", including "Global change and regional response","Restoration ecology","Biodiversity and conservation biology","Information agriculture ecology","Landscape ecology","Coastal system ecology","Ecological economics","Plant stress physiological ecology","Microbial ecology" and "Plant molecular ecology". Xinyang Tea-Forging and Development of Green Food Industry.