Detoxification Technology of Potato

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Potatoes are nutritious and resistant to storage. It has a short growth period and great potential to increase production, so it is fond of eating for people. However, potatoes are easy to be infected with a variety of viruses, resulting in smaller tubers, deformities, seed potato degradation and so on. The experimental results show that the detoxification of potato by stem tip tissue culture combined with virus detection and the production of virus-free seed potato can effectively prevent the degradation of seed potato and greatly increase the yield of potato. The main detoxification methods are as follows:

1. The tubers of the virus-free varieties were sprouted by sampling and disinfection. When the buds were 4-5cm, cut the buds and peel off the outer leaves, rinse 40min under tap water, disinfect them with bleach solution in the sterile room, and rinse with aseptic water for 2-3 times.

two。 The stem tip of the original base with one leaf was stripped and inoculated in the test tube of the MS stem tip culture base under a 40-fold dissection microscope. The MS shoot tip medium in the test tube consists of a large number of elements, trace elements, organic components and auxin, cytokinin, sucrose and Agar. The pH value is 5.7. after high pressure sterilization, one stem tip is inoculated in each test tube.

3. Under the culture conditions, the shoot tips inoculated were cultured in the room under 25C and 1500-3000LX light, and grew into plantlets with 3-4 leaves in 3 months. Under aseptic condition, cut and propagate once, and some seedlings were taken for virus detection.

The picture comes from the Internet.

4. Virus detection is an indispensable step for stem tip detoxification. Identification hosts, that is, indicator plants or serological methods are often used for detection to eliminate stem tip seedlings with positive serological reaction or symptoms on indicative plants in time. Stem tip seedlings without any reaction are used for reproduction.