Control methods of Ganoderma lucidum green Trichoderma

Green Trichoderma occurs with the highest frequency and harm, and can occur in all stages of Ganoderma lucidum cultivation. It can be described as devastating and catastrophic diseases and miscellaneous bacteria, seriously affecting the yield and quality of Ganoderma lucidum, and is the focus of disease control in Ganoderma lucidum cultivation. Let's take a look at the control methods of Ganoderma lucidum green Trichoderma.

Morphological characteristics of Trichoderma viride from Ganoderma lucidum

Trichoderma viride is a slender, colorless, septate, much branched hyphae. The conidiophores emerge from the lateral branches of the hyphae, opposite or alternate, generally branching 2-3 times, and the pedicels of conidia are bottle-shaped or conical. Most of the conidia are spherical, and the spore walls are verrucous, green or turquoise. On PDA medium, the colony was white flocculent at first, and then dark green.

Symptoms caused by Ganoderma lucidum and Trichoderma viride

Trichoderma viride widely exists in all kinds of organic matter and soil in nature, and often floats in the air in the form of conidia. It has strong adaptability, especially on the substrate rich in lignin and cellulose. Once infected, it grows and develops rapidly, the whole material layer shows a green and secretes toxins, destroys the hyphae and cytoplasm of the host, and inhibits the growth of Ganoderma lucidum hyphae. Compete with the mycelium of Ganoderma lucidum for nourishment, consume nutrients and make the host die quickly.

Incidence regularity of Trichoderma viride in Ganoderma lucidum

1. Route of transmission: conidia are transmitted by airflow, water, insects and so on. Old mushroom houses, germ-carrying tools and raw materials are the main sources of primary infection. The conidia produced after the disease can be eroded repeatedly.

2. Occurrence conditions: widely distributed in soil, air and all kinds of organic matter in nature, it is easy to be infected if the culture material is not sterilized thoroughly or the fermentation is not good. The spore germination ability of Trichoderma viride was the highest at 15: 30 ℃, the hyphae could grow in the range of 4: 42 ℃, and the fastest growth was at 25: 30 ℃. High temperature environment is beneficial to spore germination and mycelium growth. Like slightly acidic environment, but pH above 7 does not die.

Control methods of Ganoderma lucidum green Trichoderma

The main results are as follows: 1. The selection of Ganoderma lucidum varieties with good impurity resistance and strong mycelial growth potential, and the selection of high quality Ganoderma lucidum varieties is the key to successful cultivation. The varieties with good disease resistance and strong growth potential are not easy to be infected by Trichoderma viride.

2. Strictly select the species for cultivation. The selected strains require pure species, strong mycelium vitality, white, dense, robust and suitable age, so as to prevent the strain from being brought into Trichoderma viride.

3. To do a good job in the cleanliness and hygiene of the cultivation environment, the mushroom room should remove the bacterial residue and garbage, thoroughly clean the cultivation shelves, and carry out space disinfection to eliminate the hiding places of miscellaneous bacteria, so as to reduce the transmission media. Doing a good job of environmental sanitation can achieve twice the result with half the effort in preventing pollution.

4. The culture material should be fresh and mildew-free, it should be exposed to the sun for several days before use, the formula of the culture material should be reasonable, the main material and auxiliary materials should be fully mixed, the water content should be controlled at about 60-70%, the loading quantity should be appropriate, and the tightness should be moderate. sterilization under high pressure or atmospheric pressure immediately after installation to prevent acidification of the culture medium. Sterilization requires thorough sterilization.

5. Establish a strict aseptic concept in vaccination. As there are green Trichoderma spores floating everywhere in the air, the operation should not be careless because they are invisible to the naked eye. The hands, clothing, inoculation tools and materials used by the operators should be strictly disinfected. For example, operators who choose to inoculate in the inoculation room should wear hats to prevent green Trichoderma spores from falling on their hair. The inoculation action should be as fast and skillful as possible, prevent miscellaneous bacteria and spores from being brought into the inoculation process, seal the bags damaged in the sterilization process with adhesive tape, and disinfect them with 75% alcohol at the sealing place.

6. Properly increasing the amount of inoculation can make the hyphae of Ganoderma lucidum occupy the site quickly with absolute superiority, reduce the pollution of miscellaneous bacteria, and play the role of bacteriostasis of mushrooms.

7. Ensure that the culture room has a suitable microclimate, close the mycelium culture, control the temperature of about 25 ℃ and 60% humidity, pay attention to ventilation, strictly prevent high temperature and humidity, create the most suitable environmental conditions for the growth of Ganoderma lucidum mycelium, promote the rapid growth of Ganoderma lucidum mycelium, and quickly occupy the whole material surface.

8. After the primordium of Ganoderma lucidum grows, remove the cotton plug or open the bag in time to avoid damage to the primordium and infection with Trichoderma viride. Do a good job of heat preservation and moisture preservation, at the same time strengthen the ventilation of the Phoenix and give a certain amount of light to promote the primordium to grow healthily into a fruiting body, and the fruiting body will be picked in time after maturity.