Cultivation and propagation techniques of Rehmannia glutinosa

Rehmannia glutinosa is a perennial herb of Scrophulariaceae, and it is one of the precious traditional Chinese medicines in China. Because it contains Rehmannia glutinosa, cardiotonic glycosides, a variety of organic acids, vitamins, sugars and so on, it has cardiotonic, diuretic, hypoglycemic and liver protective effects. Among them, the rhizome of Rehmannia glutinosa can be obtained by different processing methods, which belongs to heat-clearing and blood-cooling drugs. Shengdi has the effect of nourishing yin and clearing heat, cooling blood and stopping bleeding, mainly treating febrile irritability, yin deficiency and low fever, hematemesis, hematuria, metrorrhagia, etc.; cooked land can nourish yin and replenish blood, mainly treat yin deficiency and blood deficiency, dizziness and tinnitus, waist and knee soreness, spermatorrhea, amenorrhea and so on. Refreshing and nourishing beverages containing Rehmannia glutinosa, Rehmannia glutinosa, Rehmannia glutinosa tea, canned Rehmannia glutinosa and ten spices of Rehmannia glutinosa pickled with fresh Rehmannia are all very popular in the market.

At present, the reproduction of Rehmannia glutinosa is a long-term vegetative propagation with rhizome, so there are some problems in the process of planting, such as large amount of materials, virus infection, variety degradation and so on, so that the resources of Rehmannia glutinosa have been seriously destroyed. resulting in a large gap in the production and supply of high-quality Radix Rehmanniae.

Collection and treatment of explants

In autumn (September to October), the strong rhizomes of Rehmannia glutinosa with a diameter of about 1.0 cm and free of diseases and insect pests were dug, washed with detergent and tap water, and cut into root segments with a length of 1.0-1.5 cm. Rinse with 75% alcohol on the super-clean worktable for 30 seconds, 0.1 liters of mercury, disinfect for 7 minutes and 8 min, and rinse with sterile water for 5 times for 6 times.

Induce the growth and differentiation of explants

The disinfected explants were used sterilized scissors or scalpels to remove the cross sections that had come into contact with the disinfectant to expose fresh tissue. The root segments were inoculated on the medium MS 6-BA 0.052.0 mg/L NAA 0.010.5mg/L in time. Be careful not to face up at the bottom. The inoculated materials were cultured at a temperature of about 24 ℃, a light intensity of 12 hours and a light intensity of 1500 lx. One week after inoculation, yellow-green buds began to appear on the roots, adventitious buds grew about 15 days, and buds grew to 2.0-3.0 cm after 30 days and 40 days.

Subculture and proliferation

The above buds were removed and inoculated on the medium MS6-BA1.5 mg/LNAAO.1 mg/L. After 10 days, there were 5 buds at the base of the shoots, and the new buds grew to about 1.0 × 2.0 cm after 20 days. After subculture every 30 days or so, the proliferation coefficient was controlled at about 3 times, and the strong seedlings were obtained while keeping the good proliferation of the buds.

Rooting and transplanting

The rootless seedlings with strong growth up to 3.0cm were divided into individual plants and inoculated on the medium 1/2MS NAA 0.05mg/L. Generally, a large number of roots could be produced after 15 days. When the root length is about 1.0 cm, it can be domesticated and transplanted. The method is to uncover the test-tube plantlets in a glass greenhouse with a temperature of 25 ℃ and a humidity of 75% for 3 days, then gently remove the seedlings from the bottle with tweezers, wash the residual Agar medium with chlorothalonil diluted 0.125% to 0.142%, and then transplant them to the disinfected substrate (perlite: vermiculite = 1:1). After transplanting, the temperature was kept at 2425 ℃ and the humidity was more than 80%, and the survival rate was more than 90%.