Matters needing attention in collecting blood from chickens

Matters needing attention when collecting blood for chickens, make on-the-spot records when collecting blood, fill in blood collection forms regularly, including the name of chicken farm, breed of chickens, day age, number of samples, immunity and other items, keep a good record of each sample, strictly prevent loss, confusion and pollution, the amount of blood collected should be taken in accordance with the testing requirements.

? Sample collection

It should be in accordance with the principles of representativeness, accuracy, rationality and scientificality. generally, at least 15 samples should be collected from a batch of chickens. The samples collected should involve different henhouses, and blood samples should be collected in the four corners and middle positions of the same henhouse, so as to be convincing.

The waste left by blood collection shall be incinerated centrally or buried innocuously.

? Preservation and delivery of blood samples

After collecting the blood, the needle should be dropped immediately, and the blood of the syringe should be injected slowly along the wall of the test tube, without air bubbles or oscillation. Number one by one, check carefully, tilt. The serum can be precipitated when the blood sample is placed at room temperature in summer and placed in 25-37 warm water for 1 hour in winter, and the serum needs to be cryopreserved. When collecting and making chicken red blood cell suspension, 2ml anticoagulant should be put into the 5ml syringe, and the anticoagulant should be dissolved in 1ml distilled water with 3.8g trisodium citrate.

When the blood sample is submitted for examination, an appropriate amount of ice should be added to the incubator to prevent the blood from deteriorating, and the violent oscillation should be avoided as far as possible. Bao Jinhua