Three measures for breeding High quality Pleurotus ostreatus

Denominator species, original species and cultivated species. They are usually called first-class species, second-class species and third-class species, and their cultivation methods are different. The main technical points are described as follows:

1. Maternal culture: refers to the bacteria obtained by tissue isolation or spore isolation. The mother culture medium is usually prepared with potato and cabbage medium. Prepare 1000 ml medium with 200g potatoes, 18-20g Agar and 20g glucose.

Practice: first wash and peel the potatoes, cut into small pieces of broad beans, boil in 1000 milliliters of water for 30 minutes, filter with 6-8 layers of gauze to make up for the loss of water. Add Agar to dissolve, add dissolved sugar and replenish water to 1000 ml. After the test tube is packed separately, the quantity of each tube is 1x5-1x4, and cotton stopper and paper wrap are added. Sterilize under 1.2 kg pressure for 30 minutes and place it on a bevel while it is hot. After cooling, it can be inoculated without miscellaneous bacteria under the condition of 25 ℃-27 ℃. A mother can turn 25-30 tubes.

When the test tube inoculated under aseptic operation was cultured at a constant temperature of about 25 ℃, the mycelium could grow full of the test tube in about half a month. In the process of culture, attention should be paid to the removal of test tubes contaminated by miscellaneous bacteria. The cultured mother seeds were transferred to the refrigerator and stored at 4 ℃ for half a year or at room temperature for three months.

2. Original seed culture: sawdust or cottonseed husk can be used as raw materials. Sawdust medium formula: sawdust 78%, wheat bran or rice bran 20%, sugar 1%, gypsum 1%. Cottonseed skin medium formula: 50 kg cottonseed peel plus potato extract juice 3%, (1.5 kg potato cut into mud and boiled filtrate for 30 minutes), sugar 0.1%, magnesium sulfate 0.1%.

Practice: mix the culture material evenly with water, hold the material tightly with your hands in humidity, and it is appropriate to see water droplets in the fingers. The general ratio of material to water is 1.2-1.3. Then bottling, the bottling method is to shake the bottle gently while filling, so that the upper and lower parts of the bottle are tight and moderate, flatten the material to the shoulder of the bottle, wash the culture material inside and outside the mouth of the bottle, add cotton stopper and wrap it into a high-pressure disinfector and put it into a high-pressure disinfector for sterilization. Sterilization at 1.5 kg pressure for one hour. After cooling, they were inoculated under aseptic operating conditions. When the mother seed in the test tube is inserted into the bottle, 4-5 bottles of the original seed can be received by a mother seed, and the mycelium can grow full in the bottle for about a month after inoculation under the constant temperature of 25 ℃.

3. Cultivation of cultivated species: the preparation of cultivated species is actually an expanded cultivation of the original species. The formula of sawdust culture medium of cultivated species is the same as that of original seed culture. The formula of cottonseed skin culture medium is also the same as that of original seed culture. Sterilize after bottling, and insert the original strain into the cultivated seed bottle under aseptic condition. A bottle of 750 ml of original seed can be used for 80-100 bottles of cultivated species. After inoculation, it was incubated at a constant temperature of 25 ℃ for about one month, and the seeds could be sown after the bottle was full. When cultivating the original and cultivated species, it should be checked every 2-3 days after inoculation, until the mycelium is full of material surface once a week. Pick out the bacteria contaminated by miscellaneous bacteria and dispose of them away from the culture room.