Three measures to breed high quality Pleurotus ostreatus strains

Denominator species, stock species and cultivars. Usually also known as first-class species, second-class species, third-class species, its cultivation methods are different, the technical points are described as follows:

1, mother seed culture: refers to the use of tissue isolation or spore isolation of strains. The preparation of mother seed medium usually uses potato agar medium. 1000 ml culture medium was prepared by 200 g potato, 18-20 g agar and 20 g glucose.

Practice: First wash and peel potatoes, cut into small pieces of broad beans, put 1000 ml of water to boil for 30 minutes, filter with 6-8 layers of gauze to make up for the loss of water. Add agar to dissolve, add dissolved sugar, and replenish water to 1000 ml. After that, the test tubes are divided into 1/5-1/4 of each tube, and cotton plugs and wrapping paper are added. Sterilize at 1.2 kg pressure for 30 minutes and slant while hot. After cooling, place it at 25 - 27 ℃ to observe the absence of miscellaneous bacteria before inoculation. A mother seed can be transferred to 25-30 tubes.

Under aseptic operation, the inoculated test tubes were cultured at 25℃, and the hyphae could grow in the test tubes in half a month. In the process of cultivation, attention should be paid to removing test tubes contaminated by miscellaneous bacteria. Cultured mother seeds were transferred to refrigerator and stored at 4℃ for half a year or at room temperature for three months.

2, seed culture: raw materials available sawdust or cottonseed hull. The formula of sawdust culture medium: sawdust 78%, wheat bran or rice bran 20%, sugar 1%, gypsum 1%. Cottonseed hull medium formula: 50 kg cottonseed hull plus potato extract 3%(1.5 kg potato mashed boiled for 30 minutes filtrate), sugar 0.1%, magnesium sulfate 0.1%.

Practice: culture medium mixed with water evenly, humidity to hand grip material, finger see water droplets and drop down is appropriate, general material water ratio 1:1.2-1.3. and then bottling, wherein the bottling method comprises the following steps of: gently shaking the bottle while loading, making the upper part and the lower part of the bottle compact and moderate, loading the bottle to the shoulder part of the bottle, flattening the material, washing the culture material inside and outside the bottle opening, adding a cotton plug, wrapping the cotton plug and wrapping the cotton plug, putting the cotton plug and the cotton plug into an autoclave for sterilization, and sterilizing for one hour under the pressure of 1.5 kilograms. After cooling, inoculate under aseptic conditions. The mother seed in the test tube is inoculated into the bottle, and one mother seed can be inoculated into 4-5 bottles of original seed. After inoculation, the hyphae can be cultured at a constant temperature of 25 DEG C for about one month, and the bottle can be filled with hyphae, which is the original seed.

3. Cultivation of cultivated seeds: The preparation of cultivated seeds is actually an expanded cultivation of the original seeds. The formula of sawdust culture medium of cultivated seed is the same as that of original seed culture. The formula of cottonseed hull culture medium is also the same as that of stock culture. after bottling, sterilizing, and inoculating the original strain into a culture seed bottle under aseptic conditions. A bottle of 750 ml seed can be connected to 80-100 bottles of seed cultivation. after inoculation, the seeds are cultured at a constant temperature of 25 DEG C for about one month, and the seeds can be sown after the bottle is full. In the cultivation of original and cultivated seeds, check every 2-3 days after inoculation until the mycelium grows to the full surface. Check once a week. Pick out bacteria contaminated with miscellaneous bacteria and dispose of them away from the incubator.