Three key points should be grasped in the cultivation of Cordyceps militaris

I. Sterilization

Sterilization can usually be divided into atmospheric pressure and high pressure sterilization to remove all microorganisms.

Atmospheric pressure sterilization generally uses an ordinary steamer, the temperature will not exceed 100℃, its cost is low, it is economical and practical, but it takes a long time to sterilize, the water in the pot needs to be steamed for at least 8-10 hours after boiling, and the fuel consumption is high, and we should pay attention to the water level in the pot at any time and add water at any time to prevent the pan from drying. High-pressure sterilization is the use of high-temperature steam sterilization pot, its cost is high, the sterilization time is not long. After automatic jet, keep 1.5-2kg / cm and the corresponding temperature is about 126℃ for 1 hour. No matter atmospheric pressure or high pressure, we must grasp the time, the holding time should not be too short, and the density of the bottles in the pot must be arranged reasonably to achieve the smooth steam in order to achieve the effect of complete sterilization.

Second, vaccination clearance

As a Cordyceps sinensis producer, we must first know what is the concept of aseptic operation, and complete the relevant procedures under the condition of excluding all other microbial interference except the target bacteria, in order to ensure the culture process of the target bacteria, that is, aseptic operation. The specific points are as follows:

First, the whole operation is carried out in sterile equipment such as inoculation box or inoculation room, and the inoculation space must be thoroughly disinfected.

Second, the space exposed by bacteria must be aseptic.

Third, all kinds of tools and bacteria before contact, must be burned by alcohol flame sterilization, cooling before contact with bacteria;

Fourth, the operator should do all the disinfection work well before carrying out the operation.

Fifth, each operation time should not be too long, so as not to increase the number of miscellaneous bacteria caused by gas exchange in the inoculation area.

Third, temperature and humidity off

Temperature and humidity are generally divided into two stages, namely mycelium production stage and fruiting body growth stage. In the mycelium growth stage of Cordyceps militaris, the suitable temperature is 5-30 ℃, the most suitable is 18-25 ℃, and the air relative humidity is 65-70%. In the growth and development stage of fruiting body, the suitable temperature was 15-26 ℃, and the optimum temperature was 20-22 ℃. It is best not to exceed 28 ℃, requiring air relative humidity of 85-95%, ways to increase humidity, watering to the ground, spraying water, air spray, and so on. Cut off the bottle film by mistake and spray water and spray directly to the fruiting body.