Medicinal resources of Cortex moutan, seed seedlings and livestock breeding

Medicinal resources of Cortex moutan, seed seedlings and livestock breeding

(1) methods and materials of reproduction

The reproduction of peony is divided into sexual reproduction and asexual reproduction. The breeding methods are different and the germplasm materials are different.

(1) Seeds are used as germplasm materials for sexual reproduction.

(2) asexual reproduction includes ramet, grafting, cuttage, striping and tissue culture. ① ramet uses several small peony trees as germplasm materials; ② grafting uses 6-10 cm annual strong branches, scions with strong terminal buds and 2-3 lateral buds growing in the lower part of peony as germplasm materials; ③ cuttings use current-year soil shoots from peony roots or stems from strong, full terminal buds, disease-free branches as germplasm materials; ④ striping uses peony branches as germplasm materials The embryos, flower buds, stem tips and tender leaves of tree peony were used as materials in ⑤ tissue culture.

(2) Propagation techniques

The sexual reproduction of tree peony, also known as seed reproduction, is a way of reproduction by using the seeds formed by ovule fertilization in the stage of sexual development. Asexual reproduction includes cutting, ramet, grafting, striping, tissue culture and other ways, the specific methods of propagation are listed as follows.

(2) grafting peony should be selected after early autumn before Chongyang, and the most suitable time is about White Dew, when the survival rate of grafting is as high as 80% ~ 90%. The root of Paeonia lactiflora and peony seedlings can be selected as rootstocks during grafting, and the scions with strong terminal buds and 2-3 lateral buds growing in the lower part of peony can be selected. Peony grafting is usually in the form of branch grafting and bud grafting. The grafting method can be divided into excavation method and resident method.

① grafting method

Dig and join method: Paeonia lactiflora root or peony root can be selected as rootstock. First, the two sides of the scion were symmetrically cut into a wedge with a knife at the lower end of the scion, and at the same time, the top of the rootstock was flattened, and then a crack with a depth of 2 3cm and a length of 3-4cm was cut from one side from top to bottom. Then the scion is inserted into the crack to make the two layers close together; tie it tightly with hemp rope or plastic film strip, smear the interface with clay slurry, and then plant.

Residential grafting method: in order to cut off all the branches above 5-6cm of the aboveground part of the original tree peony, the rest method is the same as digging grafting method.

② bud grafting method: generally choose between June and July, when the survival rate is higher. The seedlings of tree peony were selected as rootstocks, and the full buds on the branches of the same year were used as scions. Cut one knife each at about 2cm below the axillary bud of the plant, peel around or cut the square bud as the grafting bud; then cut the same size section on the same thickness of the rootstock branch, then stick the grafting bud on the section and tie it with hemp rope or plastic film strip.

The suitable time for ③ grafting of peony: the grafting of peony depends on specific temperature and humidity, and the grafting is after the beginning of autumn and before Chongyang, especially before and after White Dew.

(3) after cutting propagation before and after the Autumn Equinox, the two-year-old stout and full branches were cut into cuttings with 10~15cm length, and then the gibberellin (GA3) strips of 500-1000mg/L were inserted into sandy loam with appropriate humidity. If the strong and sprouting branches of the same year are selected, they will be cut or treated with .500 mg/L of indoleacetic acid (IAA) around September, and the branches of 2 + 3 will be inserted into the sandy loam with appropriate humidity. If the robust sprouting branches of the same year were selected, they would be cut into cuttings with 2-3 buds around September, and then cut in the mixed medium with 500mg/L 's naphthalene acetic acid (NAA) or 300mg/L 's potassium indolebutyrate (IBA). Adventitious roots could be produced when cultured at 18-25 ℃ for 20-30 days.

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