Conditions and equipment of plant tissue culture

Plant tissue culture is the cultivation of plant materials under strict aseptic conditions, so certain laboratory conditions are required. Regardless of the level of the culture room, it is generally composed of the following parts:

1. Washroom: a large number of glassware used for tissue culture are washed and placed, requiring water supply and drainage facilities.

2. Culture medium preparation room (or chemical laboratory): it is the place where the culture medium is prepared. It is necessary to have all kinds of comparative drugs, various measuring tubes, straws, test tubes and other glassware; weighing scales; refrigerators for storing mother liquor, etc.

3. High-pressure disinfection room: after the preparation of the culture medium, high-pressure disinfection is needed to kill the contaminated bacteria, so it is necessary to be equipped with a high-pressure disinfector, which requires safe and convenient electricity and heat supply.

4. Inoculation room: it is an important operation room for plant material separation and inoculation and culture material transfer. It is required to be flat and clean, so it is convenient for cleaning and sterilization. If you have the conditions, you should buy a super-clean worktable, and the simple one can also be replaced by an aseptic inoculation box.

5. The culture room: it is the place where the inoculated materials are used for culture. Its size depends on the need, usually placing a culture rack. Room temperature should not be too high and too low, conditional should install air conditioning, install fluorescent lamps to solve lighting, can also use natural light, in order to reduce energy consumption.

According to the arrangement of the above parts, the main equipment of plant tissue culture are: glassware, refrigerator, general balance and analytical balance, high pressure disinfector, super clean table or inoculation box, culture rack and so on. (Wang Huaiyu)