How to establish a training system under family conditions

The working conditions for the establishment of orchid rapid propagation system are not unattainable. Amateurs can do all or most of the operations and cultivate orchid test-tube plants by themselves.

1. Minimum essential supplies and venues

Without the most basic conditions and supplies, tissue culture cannot be achieved. Some of these necessities need to be purchased, and some can be self-made.

1. Necessities

(l) A household pressure cooker, which is used for high pressure sterilization such as culture medium, sterile water, etc. It is better to have a medical portable high pressure sterilizer.

(2) one inoculation box (aseptic inoculation box), which is made (see below for methods and requirements)

(3) the volume of an ordinary balance is 100 grams.

(4) stainless steel or aluminum pot for cooking culture medium with a capacity of 1 to 2 liters.

(5) various kinds of bottles and test tubes for culture

(6) an alcohol lamp, which can be used to burn out wormwood during inoculation.

(7) 20~25Cm long tweezers for inoculation.

(8) 1 to 2 scalpels, several blades, for inoculation

(9) 5-10 small plates for inoculation

(10) several bottles of solution

(11) one pipette for each of the 5ml pipette

(12) one for each of 1010 50100500ml.

(13) A fruit juicer to extract natural fruit juice as culture medium.

(14) skimmed cotton, rubber band, Kraft paper, etc.

With the above items, you can basically carry out the work.

Household pressure cooker has no pressure gauge, do not add valve during sterilization, boil until boiling, add valve 10 minutes later, valve jet timing, 15-20 minutes flameout, slowly deflate, sterilization is complete.

2. Essential drugs and water consumption

There is no need to put too much emphasis on purity when using water, because the culture medium is only similar to the function of soil in a sense. As long as there are few impurities, such as clean river water, well water, stream water and tap water.

Essential drugs only need to buy the most necessary, most commonly used, large consumption of a few.

(l) A large number of elements in MS medium, (a total of 5 drugs)

(2) ordinary alcohol (for lamp and surface sterilization)

(3) KOH and NaoH,Hcl for adjusting PH value, precision PH test strip (PH5.4~7.0)

(4) one bottle of bleach or bleach powder

(5) Agar

(6) White sugar

(7) 1 bottle of formalin and 1 bottle of potassium permanganate, the dosage of other drugs is very small, and they need to be weighed by analytical balance.

If it has been prepared, you can purchase the sub-packaged products from the relevant research or production departments and store them in the household refrigerator.

In the culture of orchids, the KyotO formula is commonly used, the medicine is more simple, only need to buy Huabao No. 1 Lig2 package, peptone or tryptone, you can avoid the trouble of preparing mS mother liquor.

3. How to cultivate

For aseptic sowing or culture of orchids in spare time, we should make full use of natural conditions. When inoculated, the culture bottle needs to be placed in a place where there is no direct sunlight, but strong scattered light is needed. Cover it with a cardboard box during dark culture. You can also use wood strips and glass to make a small light incubator. When the temperature is low in winter, put in one or two light bulbs to increase the temperature.

4. Self-made inoculation box

With a few materials, you can make a regular inoculation box. Materials can be made of wood, plywood, glass or plexiglass, etc. Size depends on the actual needs, generally recognized as 600~700mm, wide 400~450mm, high 500mm. There are two holes on the front that reach in and operate. Oil sleeve is installed inside. There's a removable lid outside. On the top of the inside, it is best to install an 820w firewood lamp for sterilization. Then install an alcohol lamp, so there is a 10cm vent on the lower right and left side of the back of the box, the lower hole is covered with 5'6 layers of gauze (through the air hole), and the upper hole is covered with only 2 / 3 layers of gauze (vent) to facilitate air circulation. Otherwise, due to the narrow space in the box and lack of oxygen, the alcohol will soon go out. In fumigation and sterilization, all ventilation channels should be closed and sealed. The method of fumigation and sterilization is to put 2-4 grams of potassium permanganate in a small jar and pour 10~20ml formalin into the bottle 2-10 hours before each use. That is, the reaction occurs, and formalin gas pervades the space in the box, which plays a role in sterilization. Take out the vial before operation. If you can cooperate with ultraviolet lamp sterilization, the effect is better and the use is safe.