The rapid propagation of Cymbidium has achieved initial results.

Since the 1970s, it has been reported that scientific research institutes have used bioengineering technology to propagate orchids in Orchidaceae plants, and its effect is not comparable to that of traditional methods, but this advanced technology has not been popularized due to various reasons. With the question of whether the work can be carried out in the family environment for two years, the author has obtained a small batch of test-tube plantlets of Cymbidium. Now the specific methods will be introduced for orchid friends' reference.

First, the establishment of a small family organization training room, self-made equipment simple local method to launch. It is well known that the work should be carried out under aseptic conditions. For this reason, a well-sealed glass inoculation box and air purification device were developed. after taking various technical measures, the pollution rate of rotating bottles was greatly reduced. According to statistics, the pollution rate was 44.8% from June 26 to August 26, 1994. after switching to alcohol spray to reduce dust, the success rate increased to 92% from October 4 to November 26, 1994, which won time for screening the best medium formula and laid a good foundation.

Second, how can aseptic operation be achieved in a family environment? The author focuses on the following aspects.

1. All the utensils in contact with the culture medium can be treated with dry heat method (140℃ ~ 150℃), and the utensils which are not resistant to high temperature can be sterilized by chemical method.

2. In order to solve the problem of inoculation on the flame (because there is almost no air in the box), an electric furnace of 300W was added, but it was easy to burn the explant because of the high temperature. A facility for cooling the wind.

3. In order to reduce the second pollution of the tool in the environment, after high temperature disinfection, it is placed in a closed Jane at one end, and at the same time, the purified air is constantly pumped into the bottle to cool it to ensure that the explant enters the nutrient bottle safely.

4. All items entering the box should be disinfected with alcohol before they are put in. Those who carry bacteria are not allowed to enter the box. The disinfection procedure of the box is ultraviolet disinfection on alcohol spray days, and regular fumigation with formaldehyde and permanganate.

Third, select the appropriate formula of culture medium. Induction, differentiation and rooting were carried out with Macros group plus tea acetic acid and cytokinin (6BA). However, the composition needs to be adjusted in different growth stages, especially because the rooting of orchid is more difficult than that of other flowers, the method of soaking in high concentration NAA for several seconds has a good effect.

Fourth, the adoption and disinfection of explants. Explants with long roots of seedlings (unspread leaves), along with the inoculation is the best, the survival rate is higher (the inoculation season is the best from October to February), the explant disinfection author adopts O.1 liter mercury 11%. The germicidal effect of multiple disinfection with concentrated hydrochloric acid is better.

Fifth, the training environment. Light 1000 hours 20 OOLuxMagol 10 hours, culture temperature 26 ℃ + 2 growth, air humidity should be controlled at a low level (70%-80%), otherwise it is easy to pollute again.

Here are some experiences:

First, it is feasible to set up a small tissue culture room in the family, and its investment is small (it can make full use of the conditions at home). The author spent only a few hundred yuan from the preparation to the equipment to carry out the experiment. the experimental equipment can also carry out the research of other species, such as woody, medicinal plants and so on.

Second, aseptic operation must be paid attention to ideologically, but cautiously in operation. Otherwise, the consequences of losing troops will be unimaginable.

Third, at present, because there is no solid microscope, we can only do the experiment of budding. Some measures such as cutting samples by stages and liquid plus rotating bed are proposed, which is a way to obtain a large number of protocorm-like bodies, and its propagation speed will be greatly improved.