How to detect African classical swine fever

We have summarized the topic of African classical swine fever for many times, but there are still some questions. For example, how to detect African classical swine fever? This is very important. Let's take a look at it.

I. how to detect African classical swine fever

Check the growth of all pigs every day, sample from feces, nasal juice, etc., and put them in a sterilized test tube. The spleen and lymph nodes with pathological changes or no obvious changes seen by the naked eye should be within the range of collecting samples. Then send it to the relevant quarantine station for inspection and deal with the problems in time.

II. Vaccine development

On May 24, 2019, the African classical swine fever vaccine independently developed by Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences achieved phased results. China has made five major advances in the development of classical swine fever vaccine in Africa:

One is to isolate the first African classical swine fever virus in China. The virus cell isolation and culture system and animal infection model were established, and its biological characteristics such as infectivity, pathogenicity and transmission ability were studied systematically.

The second is the creation of African classical swine fever vaccine candidates, laboratory studies have proved that two vaccine candidates have good biological safety and immune protection effect.

Third, the two candidate vaccine strains have strong genetic stability in vitro and in vivo. When the two candidate vaccine strains were continuously passaged in primary cells in vitro, there was no significant change in their biological characteristics and genome sequence, and no obvious virulence reversion was found in pigs.

Fourth, the minimum protective inoculation dose is defined, which proves that high-dose and repeated-dose vaccination is safe.

Fifth, the preliminary completion of the pre-clinical pilot product process research, the establishment of two candidate vaccine production seed bank, the preliminary completion of vaccine production seed batch purity and exogenous virus test, the preliminary optimization of cell culture and freeze-drying process of candidate vaccine.

The virus can be confirmed from the blood, tissue fluid, viscera, and other feces of infected pigs. The virus present in the blood in a low-temperature dark room can survive for six years, live for several weeks at room temperature, heat the infected blood for 55 ℃ for 30 minutes or 60 ℃ for 10 minutes, the virus will be destroyed, and many fatty solvents and disinfectants can destroy it.