Culture method of Ganoderma lucidum
Ganoderma lucidum has high medicinal value, but wild Ganoderma lucidum is rare and expensive. So, what is the culture method of Ganoderma lucidum?
First, build a shed
Choose the Ganoderma lucidum greenhouse with good ventilation, proper light, unobstructed drainage and convenient management. the shed requires clean floor, smooth walls and moisture resistance. The size of Ganoderma lucidum shed depends on the number of culture materials, generally built in the woods, the front and back of the house in the shade, close to the water source is the most suitable location. The mushroom shed should be strictly sterilized before entering the shed, and each cubic meter of space should be sealed and fumigated with 5ml formaldehyde and 10g potassium permanganate for 24 hours. Spring seed is the best from April to May and autumn seed is the best from September to October.
2. Production of planting ingredients
Planting ingredients include 77% cottonseed hull, 10% wheat bran, 10% corn meal, 1% sugar, 1% phosphate fertilizer and 1% gypsum. After the cultivation materials are mixed, they are bagged by a bagging machine. The plastic bags are packed in polypropylene or polyethylene cylinders of 15 cm multiplied by 35 cm or 17 cm 33 cm, each containing 400 grams of dry material. Polyethylene bags were sterilized under atmospheric pressure for 10 hours and polypropylene bags were sterilized under high pressure for 2 hours. When the material was cooled to less than 30 ℃, it was inoculated in the aseptic room. Turn it up and down once a week to ensure that the temperature of the bag is balanced, increase the oxygen in the bag, promote the occurrence of bacteria, and remove the bag infected with green mold.
III. Germ management
Ganoderma lucidum is a thermophilic fungus, the best mycelial growth temperature is 26-28 ℃, the fruiting body grows best at 24-28 ℃, and the fruiting body can not develop normally below 18 ℃. During the germicidal period, the temperature in the culture room was maintained at 22: 30 ℃, and the air relative humidity was maintained at 50% and 60%. Ventilate for half an hour every day and turn the bag up and down every 5-7 days. When the mycelium is full of 2/3 solvent in the bag, move it into the culture shed, loosen the mouth of the bag, gently lift it with your hand, and leave a little gap. It is appropriate to use scattered light in the shed to avoid direct light. After 25 days and 32 days, the hyphae can be filled with material bags. The mycelium development of individual material bags is uneven and can be singled out and released.
IV. Out-of-Zhi management
When the mycelium is full of material bags, use a blade to cut both ends into round openings the size of a 50-cent coin to facilitate the production of Zhi. When leaving Zhi, the temperature of the shed is maintained at 2630 ℃, the relative humidity of the air is increased to 90% 95%, scattered light and sufficient oxygen are provided, the ground is kept in shallow water, doors and vents are opened before 8 am and 4 pm every day, and ventilation is done at 12:00 when the temperature is low. The primordium was expanded for 3 to 5 days, and when the bacterial cap was gradually formed, spray water was added to keep moisture, and when the temperature was too high, spray water to keep moisture.
V. Harvest
Stop spraying water one week before harvest, close the ventilation door, and cover the floor with plastic film to collect sporopollen. After the spores are released, the cap changes from soft to hard, with no light white edge, and the color changes from light yellow to reddish brown. When it no longer grows and thickens, mature Ganoderma lucidum can be harvested.
In addition, it should be dried and bagged in time after harvest and stored or sold in a dry room. The finished product is dry, thick cap, stout stalk, hard, reddish brown and lacquer-like luster.
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