The latest course on breeding techniques of Clematis roxburghii

The traditional propagation methods of Anoectochilus roxburghii are ramet, cuttage and seed sowing. Because it is not easy to harvest seeds and the germination rate of sowing is low, most of them were propagated by ramet and cuttage in the past, but they were easily affected by the environment. The survival rate of this method is about 30% to 70%. Large-scale planting is generally propagated by tissue culture, so let's take a look!

Ramet propagation of Clematis roxburghii

Clematis rosette propagation can be carried out in spring and autumn, generally every three years, where the plant growth is robust, pseudocorm dense can be ramified, after each clump to preserve at least 5 connected pseudocorms. Irrigation should be reduced before splitting, so that the basin soil is better than. When putting on the basin after ramet, first cover the bottom hole of the basin with broken tiles, then cover with coarse stones, occupy the depth of the basin from 5 to 1, and then put coarse-grained soil and a small amount of fine soil, and then plant it with sandy loam rich in humus. Planting depth to the false bulb just buried in the soil strength, the edge of the basin left 2 cm along the mouth, covered with green cloud grass or fine stones, and finally watered thoroughly, placed in the shade for 10-15 days, keep the soil moist, gradually reduce watering, and carry out normal maintenance.

Sowing and propagation of Clematis roxburghii

The seed of Clematis is very fine, there is only one underdeveloped embryo in the seed, the germination power is very low, and the seed coat is not easy to absorb water, so it can not germinate by conventional sowing, so it is necessary to use orchid or artificial medium to supply nutrients before it can germinate. it is best to sow the uncracked fruit, sterilize the seed with 75% alcohol, take out the seed and soak it with 10% sodium hypochlorite for 5-10 minutes. Take it out and rinse it with aseptic water for 3 times and sow it in the culture bottle containing the culture medium, then put it in the dark culture room, keep the temperature about 25C, germinate and then move to the light to form the protocorm. It takes half a year to a year from sowing to transplanting Clematis. Tissue culture has been successful, and this method can be used to propagate where possible.

Tissue culture of Clematis paniculata

1. Prepare the culture medium: prepare the culture medium according to the requirements of the specific formula, pack the prepared culture medium, put it into a high-pressure disinfector and disinfect it. The sterilized medium is either directly used or preserved in the aseptic inoculation room for use, but it should not be stored for more than two weeks.

2. Explants cut, sterilized and inoculated: under aseptic conditions, explants of appropriate length (stem tips, stem segments, leaves, buds, roots) were cut from excellent mother plants, inoculated in culture medium, and put into culture room for induction.

3. Cluster seedling induction and differentiation induction: under the light of 20: 28 ℃ in the culture room, 12: 16 hours under the fluorescent lamp of light intensity 3000~4000Lux, and under the action of high concentration of cytokinin and low concentration of auxin in the culture medium, the explants were induced to differentiate into buds.

4. Subculture and proliferation:

After the tufted buds grew in the medium for a period of time (usually 20-30 days), with the nutrient consumption of the medium, it must be transferred to the fresh medium for culture, that is, subculture. In order to expand the propagation rate and reproduction coefficient, subculture transfer can be carried out by changing the culture conditions, such as culture at lower temperature for a few days, then transferred to another higher temperature for a few days, or liquid oscillation culture for a certain period of time. Can significantly increase the rate of proliferation.

5. Rooting induction: when the shoots grow to a certain height, they are transferred to the rooting medium with higher auxin concentration to continue culture. the young seedlings are first cultured in the dark for a week, and then transferred to normal light, or shortened light, which can take root earlier and improve the rooting rate and rooting speed.

6. Transplanting seedlings: transfer rooting seedlings from the culture room to outdoor soil and cultivate them into qualified afforestation seedlings.

Wood.

① seedling classification: according to the seedling quality index, the seedlings should be graded, the first and second seedlings should be transplanted in time, and the third seedlings should continue to be cultured.

② seedling refinement: usually use the method of enhanced light to refine the seedlings for about a week, or add a retarder to the culture medium, transfer the seedlings together with the culture bottle to the greenhouse 1-2 days before transplanting, and open the bottle cork.

③ transplanting: when the root system grows to about 1 cm, carefully take out the seedlings from the culture bottle and wash off the culture medium with clean water to avoid injuring the plants. Soak the seedlings in dilute sodium sulfate solution for a few minutes before transplanting, spray through water and cover with plastic film to protect against wind and moisturize.

④ post-transplanting management: proper shading at the initial stage after transplanting to prevent direct sunlight, and then gradually increase the time and intensity of light, the temperature should be controlled at 20-30 ℃, properly watering and moisturizing, controlling soil moisture, and gradually implementing full light. When the seedlings have been fully adapted to the natural environment after a period of time, they can be transplanted into light matrix net bags and come out of the nursery when the seedlings meet the standard.