Orchids culture Orchids tissue culture video

There is a tissue culture technology of orchids in the middle!

Tissue culture is an advanced method of asexual reproduction. Take a small part of plant organs or tissues, separate from the mother and culture, after induction and differentiation, make it produce independent new plants. Also known as explant culture.

As early as 1902, German botanist Haberlandt predicted: "Plant cells are totipotent, and each cell, like an embryonic cell, can be cultured into a complete plant in vitro." Under the guidance of this hypothesis, French scientists Gautheret and Nobecourt successfully used small pieces of carrot root to culture callus in test tube in 1938. In 1939, American scientist White used tobacco stem cambium for tissue culture and subculture, which was also successful. Their work laid the foundation for the development of plant cell and tissue culture techniques. In 1949, Skooog and others differentiated seedlings from callus and cultivated real test-tube plants. Since then, the technology of tissue culture has been innovated and improved continuously, and has made rapid progress in experiment and application. The stem tip tissue culture of orchids was first successful by the Frenchman Morel in 1960. The purpose of his stem tip culture was to remove the virus from the orchid plant, and he got virus-free seedlings. He found that the stem tip culture of Cymbidium could gradually increase to form protocorm-like bodies, which were similar to the normal development of orchid embryos and could be proliferated by repeated culture. according to theoretical calculation, one explant could produce as many as 4 million seedlings in one year. Since then, tissue culture technology has been applied to the propagation of orchids, and knives have formed the industrial and commercial production of orchids for many years.

At present, all parts and organs of orchids, such as roots, stems, leaves, buds, inflorescences and immature embryos, can be cultured as explants. Tissue culture should have the necessary equipment and relatively high technical measures. First of all, there must be a culture room, aseptic inoculation room and a variety of training appliances, supplies and so on. In culture, we should first prepare the culture medium, peel off the explants, then disinfect, inoculate, transfer, and finally transplant test-tube plantlets, all of which require more complex and meticulous techniques.