What kind of growth environment is suitable for Dictyophora? How to prepare the culture medium?

Dictyophora, also known as Bamboo Sheng and Bamboo Ginseng, is rich in nutrition, rich in flavor and delicious in taste. It has been listed as one of the "eight treasures of grass" since ancient times. Let's take a look at what kind of growth environment it is suitable for. How to prepare the culture medium?

1. What kind of growing environment is suitable for Dictyophora?

Dictyophora is a typical mesophilic fungus. The growth temperature of mycelium was 4: 28 ℃, the optimum temperature was 20: 23 ℃, and the mycelium grew slowly when it was lower than 16 ℃ or higher than 36 ℃. The fruiting body formation temperature is 17-29 ℃, and the optimum temperature is 20-24 ℃, and the optimum temperature is 22 ℃; above 28 ℃, the growth is slow and the growth potential is weak; above 35 ℃, the growth stops; below 15 ℃, the development slows down and the bacteria ball shrinks or appears malformation. The temperature referred to here is the ambient temperature in which the hyphae and fruiting bodies grow, that is, the underground temperature of 5 cm to 20 cm in the culture medium and 1 cm to 30 cm in the ground where the fruiting body differentiates. Because there is a big difference between the surface temperature and the underground temperature at the same time, in general, the surface temperature is higher than the underground temperature, the deeper from the surface, the lower the temperature. We should pay attention to this when cultivating.

Second, how to prepare the medium?

1. Strain preparation

Formula and preparation of mother culture medium.

The formula of ① medium: protein 1%, glucose 1%, Agar 2%, water 100ml. The pH value is 5-6.

② potato 25%, glucose 2%, Agar 2%, water 100ml. The pH value is 5-6. The production of culture medium: according to the conventional preparation, tube, sterilization, made into bevel.

2. Original seed preparation

First, crushed bamboo (1cm) 65%, sawdust 15%, wheat bran 20%, add water to the culture material with a water content of 65%. Second, scraps 75%, wheat bran 20%, sugar 1%, gypsum 1%, soybean cake powder 3%, add water to 60% 65% of the water content of the culture material.

According to the conventional seed production methods, ingredients, bottling, sterilization, inoculation and culture. At the temperature of 18-23 ℃, the original species grew full in about 60 days, and the cultivated species grew well in 40-50 days.