Can the valuable medicinal material Cordyceps sinensis be cultivated artificially? How do you raise it? What are the market prospects?

Cordyceps sinensis is a kind of precious medicine that appeared recently, and it is a kind of bacteria cultivated by silkworm pupa. It is very popular because of its anti-aging, prevention and treatment of heart and brain thrombosis, beauty and beauty, because it is very difficult to cultivate, so it is mostly artificially cultivated, so how can Cordyceps sinensis be cultivated artificially?

1. Strain

The silkworm itself is not the natural host of Cordyceps sinensis. Therefore, in order to cultivate Cordyceps sinensis, it is necessary to screen the strains of Cordyceps sinensis with strong infectivity to silkworm and good production performance. At present, in the report of successful artificial culture of Cordyceps militaris, most of the strains used are Cordyceps militaris, but there is no report about the successful infection of Cordyceps militaris (Cordyceps sinensis) to silkworm. Cordyceps militaris is widely distributed and can be found all over the country. at present, the strains used in all relevant reports are strains with good infection to silkworms after repeated screening and artificial domestication on the basis of wild Cordyceps sinensis.

Different dosage forms of bacteria have different infection effects on silkworms. Oblique bacteria, liquid bacteria and bacterial suspension can all be used to infect silkworms. Wen Lu and other studies believe that the bacterial suspension prepared by scraping inclined bacteria with aseptic water has the best infection effect.

2. Inoculation method

Live silkworms were inoculated with ①. Cordyceps militaris is not the pathogenic strain of silkworm, and the intestinal digestive juice of silkworm is strongly alkaline, so it is difficult for Cordyceps sinensis spores to survive. Therefore, the silkworm can not be effectively infected by simple feeding or body spraying, and the silkworm can be infected only by puncture inoculation and injection inoculation with insect needle. Wen Lu believes that the effect of puncture inoculation is the best. However, the result of our experiment is that the effect of injection is obviously better than that of other methods. There is also a live silkworm inoculation method, which is to sprinkle the sterilized silkworm body on the solid bacteria, then cover the space of the worm body with the bacteria, and then cover it with a sterilized plastic film, which is compacted around, and the mycelium of Cordyceps sinensis can infect the silkworm body for about 10 days.

② was inoculated on silkworm culture medium. This method is to dry the 5th instar silkworm after high temperature steam sterilization, then add a certain amount of nutrients, inoculate the cultured bacteria on the body surface of the silkworm by tissue block method under aseptic condition, and then culture under aseptic conditions. it can also cause silkworm infection. This method is actually equivalent to using silkworm as culture medium to provide animal nutrients needed by Cordyceps sinensis.

3. Inoculation time

The 5th instar silkworm was selected as the culture medium for silkworm. During this period, the silkworm was relatively large and nutritious. The 3rd or 5th instar silkworm was selected to infect silkworm with solid bacteria. As for the best time for puncture infection of silkworm, there are different views. Some people think that the infection rate of silkworm is the highest at the 6th day of 5th instar, while others think that the inoculation of silkworm from 5th instar and 6th day of 5th instar is better. Because the silkworm is no longer dormant after the 5th instar, and it has a period of 8 ~ 9 days, which coincides with the course of the disease, which is the best age for inoculation. although the success rate of inoculation is the highest at the 6th day of the 5th instar, it can not make effective use of silk gland protein, and the nutrition and the weight of Cordyceps are affected. In a word, the silkworm of 1 ~ 4 years old is smaller, the nutrition of Cordyceps sinensis inoculated in its body is limited, and the age of each age is relatively short, the infected silkworm can not molt normally during dormancy and is easy to be infected with other pathogens. The silkworm in the later stage of 5th instar can cocoon normally before being infected to death, which not only the silk gland protein in its body is not used by Cordyceps sinensis, but also brings inconvenience to production. Only the silkworm in the early stage of 5 years old has large body and long age, which is more suitable for inoculation.

4. Disinfection methods

The disinfection methods of silkworm before inoculating cordyceps include ultraviolet disinfection and disinfectant disinfection.

5. Symptoms after infection

After the silkworm was infected with Cordyceps militaris, there were no obvious symptoms at the initial stage. At the initial stage after the larva was infected, the silkworm was soft, elastic, head and chest protruded, and there were small needle-like brown disease spots on the body surface. After 2 or 3 days, the worm body gradually hardened, the intersegmental membrane and the valve on the body surface showed white aerial hyphae, and round conidia appeared on the aerial hyphae.

Market Prospect Analysis:

Cordyceps militaris is a kind of Cordyceps, which is cultivated artificially by using silkworm larvae as culture medium. Its nutritional value is higher than that of Cordyceps militaris and similar to that of wild Cordyceps. The success of artificial cultivation of Cordyceps sinensis makes up for the insufficient supply and high price of wild Cordyceps sinensis, and provides a new way for the comprehensive utilization of sericulture in China. It is profitable to plant silkworm and cordyceps in the future.