Cultivation techniques of chicken longitudinal bacteria

Chicken fir fungus tastes delicious, is the top of the seedlings, Shanzhen is the most, is one of the famous wild edible fungi in China. It is very popular in the market, so what are the cultivation techniques of chicken fir?

First, training technology

As the raw material for the development of nutritious food and beverage, the protein content of Agaricus gallinarum is higher than that of other mushrooms, especially the contents of lysine and leucine are very high. Therefore, more attention has been paid to the production of liquid submerged culture mycelium.

Liquid medium formula suitable for medium formula:

Formula 1: protein knee 2%, sucrose 2%, magnesium sulfate 1.5%, potassium dihydrogen phosphate 0.3%, vitamin B11 mg / 100ml, pH adjusted to 6.

Formula 2: yeast extract 0.1%, sucrose 3%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 1%, sodium nitrate 0.3%, potassium chloride 0.05%.

Formula 3: yeast extract 0. 1%, sucrose 3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.5%, sodium nitrate 0.3%, potassium chloride 0.05% PH6 (Hong Zhen, China Medical University, 1992).

2. Nutrition

The main nutrient source for the growth and development of nutritious wild chicken is obtained from ant nest, fungus nursery and its surrounding environment. The ant nest is mainly formed by the semi-digestive colony of workers into a bacterial nursery, or bacterial bed, which is rich in symtrophic substances, including lignin, cellulose, fat, a variety of amino acids and ash elements. In addition, the minerals and water around the nursery constitute the natural medium for the growth and development of Fusarium gallinarum. The excreta of termites contains amino acids and soluble nitrogen-containing compounds, which provides a good nitrogen source for the mycelium of chicken, which is conducive to the growth of mycelium. According to he Sheng (1995), the ant nest is mostly built 20-100cm underground, with a volume ranging from 0.1m to 10m. There are central nests and scattered satellite nests with ant paths connected to each other, and ant colonies will multiply in the nest for a long time for several years to decades.

Third, temperature

The temperature in the termite nest of the chicken longitudinal fungus growing place is stable at 22 ℃ + 30C, the lowest is 15 ℃, the highest is not more than 28 ℃, and the temperature is almost constant. A stable temperature is beneficial to the growth of mycelium and the hatching and reproduction of termites. From the artificial domestication experiment, it was observed that the temperature range of germination was 12 ℃-24 ℃, the optimum temperature for mycelium growth was 16 ℃-20 ℃, the mycelium grew up to 0.73cm in 10 days from inoculation to full tube at 18 ℃, the temperature for fruiting body formation was 25 ℃-35 ℃, the optimum temperature was 25 ℃-30 ℃, and the daily temperature difference was 5 ℃. When the temperature was higher than 35 ℃, the fruiting body could develop as usual.

Fourth, humidity

The stable environmental conditions in the ant nest are suitable for the growth of chicken longitudinal mycelium, but not conducive to the formation of fruiting bodies. Therefore, under normal circumstances, the mycelium grows in large quantities in the ant nest without forming a fruiting body, and Rain Water needs to infiltrate into the soil around the ant nest after the rainy season, when the mycelium grows out of the ant nest and produces fruiting bodies on the surface layer. This shows the importance of water and humidity to the fungus. In artificial cultivation, the water content of the culture material is 60%, 75%, and 65% is the best; when the water content of the culture material is 70%-75%, the fruiting body is the most suitable. The air relative humidity of the mycelium growth environment is about 80%, and the air relative humidity of the protoplast formation stage should not be lower than 80%. In the fruiting body growth stage, the ambient air relative humidity is 85%, and the fruiting body needs more than 95% of the air relative humidity.

5. acidity and alkalinity

The pH of ant nest nursery is relatively stable, usually in the range of 4.04.5.Chemical analysis shows that Wang Anyuan, which is responsible for the formation of A PH, is exposed to ten organic acids, such as volatile formic acid, non-volatile aconitic acid, fumaric acid, succinic acid and so on. The main factor for the stability of pH is the buffering effect of minerals in nesting soil and plant residues of new shippers. According to he Xinsheng (1995), it is the most suitable for the mycelium growth of pH 4.04.5, but it is not conducive to the growth of bacteria and other microorganisms, especially the growth of Xanthomonas charcoal, so as to ensure the absolute dominant position of chicken longitudinal bacteria.

VI. Lighting

Light is not necessarily needed for spore germination, mycelium growth, primordium differentiation and fruiting body growth. When the fruiting body opens the umbrella, it needs some scattered light to make it differentiate and develop well.