Hatching method of cultured gecko eggs

In the wild, the hatching of gecko eggs depends on the natural temperature, and the length of the incubation period is closely related to the environmental temperature, as well as the morning and evening of the spawning period. Eggs laid before July 15 that can hatch eggs in the same year are called hatched eggs in the same year; eggs laid after July 15 that year cannot hatch larvae in the same year are called overwintering hatched eggs. The incubation period of the eggs is in summer and autumn, the temperature is suitable, the embryo develops rapidly, the embryo period is 92 to 119 days, with an average of 105.1 days, and the average accumulated temperature is 2875.65 ℃. The embryo development period of overwintering eggs entered the middle and low temperature stage of the year, and the embryo development was retarded. The embryo stage was 280,315 days, with an average of 297.5 days, and the average accumulated temperature was 5738.05 ℃. If the accumulated temperature during hibernation was removed, the embryo period of overwintering eggs was actually 116 days, with an average of 125 days, and the average accumulated temperature was 2961.9 ℃. It can be seen that except for the hibernation period, the embryo period and accumulated temperature of the hatched eggs are similar to those of the overwintering eggs.

Experiments on the characteristics of embryonic development and external factors affecting hatching showed that when the gecko embryo developed into the seventh day, the embryo could be distinguished by the naked eye, there were spots on the yolk and the appearance was dark gray, and the yolk was separated with tweezers. The obvious massive ovule can be seen, which is about 4 mm in length; on the tenth day, the head can be distinguished and the two black eye spots can be clearly seen; after 50 days of embryo development, the egg can be touched. After 91 days of embryonic development, the yolk basically disappeared or only a little remained, the embryonic development was completed, and the clam scale curled up in the eggshell.

Whether a fertilized egg can develop normally, in addition to the internal factors of the egg itself, the most important is the external environmental factors, that is, temperature and humidity. The length of the incubation period is directly related to the hatching temperature. In the temperature range of embryonic development, the incubation period is short when the temperature is high, and long when the temperature is low. The temperature of embryonic development is 20-37 ℃. The embryo development stopped below 20 ℃, the embryo development was slow at 20-25 ℃, the embryo developed rapidly at 26-37 ℃, and the embryo development died over 38 ℃. The most suitable humidity for embryonic development is 70% to 85%. Experiments show that gecko eggs can hatch with relative humidity of 50% to 90%. When the relative humidity is below 50%, the fertilized eggs will lose water and affect embryonic development. Long-term relative humidity above 90% will cause moldy fertilized eggs and affect embryonic development. Therefore, during artificial hatching, the humidity should be controlled at 70% to 85%.