Technique and Advantages and Disadvantages of Artificial Fertilization in Rex Rabbit

Artificial insemination of rabbit semen collector mainly includes semen collection, semen quality evaluation, dilution, preservation, transportation, insemination and so on. The operation of rabbit artificial insemination device is as follows:

1. Preparation before semen collection

(1) preparation of semen collector. At present, there is no unified specification and standard for semen collector, which is generally composed of false vagina and semen collector. The structure of two kinds of false vagina is introduced for reference in practice. A. simple fake vagina. Choose a hard plastic tube or bamboo tube with a diameter of 3~4cm (inner diameter 2.5 to 3 cm) as a false vaginal shell, take both ends of the inner tube to turn out a rubber ring and fix it on the shell, punch a hole in the middle of the shell to install a water injection piston, connect a glass vial or sampling tube as a seminal collector, disinfect it with 75% alcohol before use, and then rinse with normal saline. When in use, the temperature of 50 °60 °C is injected into the shell piston to about 2 °C of the space in the shell, and it is appropriate to keep the water temperature 38 ~ 40 °C when collecting semen.

(2) the preparation of inseminal apparatus. Rabbits do not have a special insemination device, and a general 5~10ml glass syringe is used as an insemination device.

(3) Disinfection of artificial insemination instruments.

(4) the training of semen collecting male rabbits need to train the male rabbits who have not collected semen, and choose healthy estrus female rabbits to let the male rabbits climb, but do not mate.

two。 Semen collection

Semen collection is usually carried out in the cage of male rabbits. The semen collector is ready to adjust the temperature to 38 ~ 40 °C, and the opening end is coated with a little Vaseline or soap liquid for lubrication. When the Shuihu breeding company uses the estrus female rabbit as the sperm collecting platform rabbit, the sperm collector grabs the female rabbit's ears and neck skin in one hand, protects the female rabbit and makes its hindquarters face the cage, the other hand holds the false vagina under the female rabbit's belly, and the opening end of the false vagina is close to the female rabbit's vagina, so that the false vagina is at an angle of 30 degrees to the horizontal plane. When the male rabbit climbs onto the back of the female rabbit after teasing, the false vagina should quickly and accurately aim at the angle and position of the vagina to facilitate penile insertion. When the buttocks of the male rabbit rush forward, the male rabbit will ejaculate, and the male rabbit will fall to the ground with the sound of "goo". At this time, the false vagina should be retracted and the opening end should be made to prevent semen from spilling, remove the semen collector and send it to the artificial insemination room for examination.

Semen collection false platform rabbit sperm collection operation is basically the same as above, but the requirement is libido or trained male rabbits. The frequency of semen collection is 1-2 times a day, and you should have a rest for 5 days.

Normal ejaculation volume 0.5~2ml, good semen is milky white, thick, generally the greater the sperm density, the whiter the color, the thicker are better semen.

3. Insemination

Estrus identification of female rabbits should be done first for insemination.

(1)。 Ovulation treatment before insemination because rabbits are irritant ovulatory animals, ovulation must be carried out before insemination. Methods each rabbit was injected with LRH-A-2 5~10ug or human chorionic gonadotropin 50IU into the middle ear vein of 0.2ml normal saline, or the male rabbit with vasectomy was used to climb, but some people thought that the effect of empty climbing to stimulate ovulation was not ideal, so semen was performed immediately after ovulation stimulation.

(2)。 During insemination, the female rabbit was first taken out and placed on the table, and the operator grasped the buttocks of the female rabbit tightly with one hand, using 75% alcohol cotton ball or iodine cotton ball to wipe off the dirt of the female rabbit's vulva and disinfecting the vagina. The other hand held the vas deferens and inserted the vas deferens near the cervical orifice of 7~9cm along the back of the vaginal wall, slowly pushed into the semen 0.3~0.5ml, and the number of pin sperm was not less than 10 million. While inserting the vas deferens, twitch the vas deferens back and forth several times to stimulate the female rabbit, pull out the vas deferens and then raise the buttocks of the female rabbit for a while and take a pat to prevent semen reflux. The effect of insemination by inverted extraction is also very good, that is, the head and neck of the female rabbit is gently sandwiched between the operator's knees, the buttocks and tail of the rabbit are grasped by hand, and the vas deferens is carried by the other hand. It can also be operated by two people, one Baoding female rabbit and the other insemination.

Advantages and disadvantages of artificial insemination of Rex rabbits:

Artificial insemination of Rex rabbit is the simplest and most effective method for breeding and commercial rabbit production. it is an important means to raise rabbits scientifically and realize the modernization of rabbit production.

(1) the disadvantage is that if the production level of the rabbit farm itself is not high and the technology is not up to standard, artificial insemination is likely to lead to an increase in uterine inflammation, low conception rate and low litter size in female rabbits. It is suggested that technicians should learn the technology first, and then carry out small-scale artificial insemination experiments, or adopt the combination of natural mating and artificial insemination, and then popularize it with the continuous improvement of production level and technology.

(2) the advantages of rabbit artificial insemination:

① can increase the male-female ratio and reduce the feeding cost. The ratio of male to female in natural mating is LRV 416, which can be expanded to 1RV 80: 120 by artificial insemination, which improves the utilization rate of improved male rabbits. Eliminate the poor male rabbits, reduce the breeding amount of male rabbits, save the cost of feed and equipment, and greatly reduce the feeding cost.

② solved the difficulty of mating. In particular, the extra-large meat rabbits are large and difficult to mate naturally, which directly affects the fertilization rate of female rabbits. The difficult problem of mating can be solved by using artificial insemination.

③ overcame the selection and mating of male and female rabbits. In natural mating, both male and female rabbits have preference, which affects the fertilization rate. Only artificial insemination can solve this kind of selective mating and improve the fertilization rate. In addition, some excellent male rabbits can not mate naturally due to trauma, especially leg injuries, and the use of artificial insemination can give full play to the utilization value of excellent male rabbits.

④ is a great reform of breeding work. Artificial insemination does not need single cage breeding and recording, which not only records accurately, but also improves the production performance of offspring and speeds up the breeding process through the detection of semen quality of male rabbits.

⑤ reduces the spread of disease. It mainly refers to the spread of mating organ diseases in male rabbits. When the mating organs of male rabbits are sick, the semen of male rabbits is contaminated, and if they mate naturally, it will lead to vaginal disease of female rabbits.

⑥ enlarges the gene pool. Semen freezing technology can make the fertilization rate reach 8090%, which is not limited by the age, time, region and national boundaries of male rabbits. no matter how long it is, excellent strains can be preserved by freezing technology, and its semen can be used to reproduce.

⑦ is easy to popularize. Artificial insemination technology is simple and easy to operate, and does not need more sophisticated and complex equipment. Generally speaking, with a junior high school education level or above, you can operate independently after 10-15 days of study and practical training.