Technical points of artificial insemination in Pigs

Preparation before insemination: the collecting cup should be placed in a 35-37 ℃ incubator, and the boar of artificial insemination does not need to carry out this intercourse. Color: normal semen is milky white or grayish white. Odor: normal semen has no special odor. PH value: the PH value of semen is generally between 7.07.8. Sperm motility and deformity rate: if the proportion of sperm moving forward is less than 60%, it can not be used. Semen dilution: diluted semen should be used within 3 days.

I. preparation of pigs before artificial insemination

1. Before collecting semen, the collecting cup should be placed in a 35-37 ℃ thermostat to avoid the impact of the temperature difference on the sperm, and direct sunlight should be avoided on the semen.

2. Boars generally used for artificial insemination do not have to carry out this intercourse to avoid affecting the libido and mood of boars during semen collection. In order to ensure the boar's libido and high-quality semen, 65% of the semen is generally changed every year.

3. The semen collected must be strictly examined. After the semen collection is finished, the semen should be put into the 37 ℃ incubator immediately, and the strict quality and artificial insemination examination should be carried out as soon as possible.

Second, observe the color

1. Generally normal semen is milky white or grayish white, semen with high concentration, usually milky white with yellowish white, low concentration semen, grayish white, water sample or even transparent.

2. If the semen is reddish brown, it may be mixed with the blood, if it is yellow and smelly, it may be mixed with urine or inflammatory secretions, such semen can not be used, at the same time, boars should be treated in time.

Third, smell

Normal semen has no special smell, semen with urine smell, ammonia smell and other strange smell can not be used.

Fourth, PH value

In general, the lower the PH value of semen, the greater the concentration of sperm. normal semen is neutral and weakly alkaline, about 7.07.8.

5. Sperm motility and deformity rate

1. The motility and deformity rate of sperm can only be observed under 200-400 times microscope. If the proportion of sperm moving forward is less than 60%, it can not be used.

2. Then the sperm density is measured by an electronic calculator, and the number of sperm in the collected semen is calculated. According to each insemination dose, there are at least 3 billion effective sperm, and the dilutable multiple is calculated, which can be diluted by 10-15 times.

Semen dilution

1. First of all, prepare the specialized commercial diluent and the self-made distilled water. The temperature of the diluent and the semen to be diluted should be the same, and the temperature difference should not exceed 1 ℃.

2. Semen can be diluted according to the dilution multiple, and the automatic mixing mixer and glass rod can be used to fully mix and stir, and then use a 100ml plastic bottle with a tip, which needs to be sub-packed with non-toxic and harmless plastic tape, marked with a boar number and semen collection time. To distinguish.

3. Finally, the diluted semen is preserved and stored in a room temperature of 25 ℃ for 1-2 hours, and then stored in a thermostat. It can be turned every 6 hours or 12 hours, and can be preserved for 5-6 days. It is generally required to be used within 3 days, otherwise the effect of fine insemination.