Cultivation techniques of Lentinus edodes with bag material

Lentinus edodes is a common edible mushroom in daily life. it is sold at about 5 yuan per jin on the market, which is more common in hot pot restaurants and spicy pot restaurants. It is being planted in many areas. What is the cultivation technology of Lentinus edodes with that bag material?

1. Producing strain

Mother seed: prepare 200g peeled potato, 20g glucose, 2g peptone, 20g Agar, 1 liter of water according to the conventional method, then disinfect the surface of the separated materials, and culture the bacterial meat the size of soybeans on the bevel medium for heat preservation. After the mycelium grows all over the slope, the mother seed will be obtained.

Original seed: prepare some wheat grains, wash and soak overnight, then cook, remove and drain, then mix 94% wheat grains, 1% calcium carbonate and 5% wheat bran into a strain bottle and compact them, plug them with cotton plugs and wrap them with moisture-proof paper. 05 kg per square centimeter pressure sterilization for 1 hour. After inoculation, cultured in 24-27 ℃ for 10 days, the hyphae could grow all over the bottle.

Cultivated species: the composition of the culture medium and the preparation method are the same as the original species.

2. Make a bacterial lump

(1) pressing: in the first and middle of October, first spread the wooden board or Reed mat on the bedstead, then pad the plastic film on it, and put it on the film to make a model. The specification of the pressing die is 65-75 × 25-35 × 5.5 cm, not the bottom plate, but with a movable cover plate. Making mushrooms is similar to making bricks.

(2) Sterilization: first open the cotton stopper of the strain bottle, wipe the mouth of the bottle with 75% alcohol and sterilize, and then use the inoculation rake to take out the strain and pour it into the frame. Try not to damage the hyphae when controlling the bottle and keep it in pieces. Each clot can be poured into about 10-12 bottles of bacteria. Then cover the cover plate and press it into a bacterial lump. The four corners of the block should put more bacteria, so that when pressing the block, the four corners of the block will be more solid, and it will not be easy to break when you touch the corner with your hands. Each piece should be kept at a distance of 1 cm to 2 cm to facilitate operation, ventilation and control of miscellaneous bacteria infection. After the fungus is filled with a bedstead, cover it with plastic film in time. The covered film should have a space of 16 cm from the bacterial mass.

3. Bacterial mass management

Always pay attention to the ventilation in the bacterial bed and open the doors and windows regularly every day. The plastic film should be opened for ventilation 3-5 days after pressing, and the frequency of air exchange should be determined according to the air temperature. The temperature is high, once in the morning and evening; if the temperature is low, change once a day or once every two days. In this process, if you find red stagnant water in the bottom plastic film, you should wipe it dry in time. Generally speaking, about 20 days after pressing, the whole mycelium can be covered with white mycelium and form a solid clump, which can produce mushrooms at this time.

4. Management of mushroom production period

(1) Autumn mushroom management: when the buds grow to the size of soybeans, the fungus should be turned over in time. If there are buds on the top or bottom of the bacterial block, you can also cut the bacterial block small so that the one with more buds is facing up, and then raise or remove the plastic film for moisturizing treatment, so that the water content of the bacterial mass reaches about 55-60%. The relative humidity of the air should reach 85-95%. In this way, the buds can develop normally.

(2) Management after mushroom harvest: after the mushroom is collected, the water spraying should be stopped, the water content of the mushroom mass should be gradually reduced, and the ventilation should be strengthened to reduce the relative humidity of the air to about 75%, resulting in environmental conditions conducive to the growth and development of the mycelium. In order to facilitate the second batch of mushrooms.

(3) Winter management: the main thing in winter is to preserve the hyphae, but the temperature sometimes rises in winter, and there is a large temperature difference sooner or later, which is a good time to get more mushrooms. After the mushroom harvest, stop spraying water, and then carry on the appropriate heat preservation treatment, such as covered with plastic film and so on.

(4) Spring mushroom management: at the end of February or early March, the fungus should be kept moist and allowed to produce mushrooms. If a small amount of buds are found in some bacterial blocks, they will begin to soak in water to promote mushrooms, which can achieve good results. If you encounter a cold wave after being immersed in water, you can heat up manually as appropriate, and this batch of mushrooms are generally neat. After mushroom, the nutrition of the fungus has been exhausted, whether it can be reused or not can be dealt with as appropriate.