How to use Mulberry branches to cultivate Ganoderma lucidum

The artificial cultivation technical process of mulberry branch Ganoderma lucidum is as follows: Mulberry branch collection-cutting-crushing-ingredient-bagging-sterilization-cultivation management-collecting Ganoderma lucidum-drying-packaging.

The main results are as follows: 1. Mulberry branches are cut and crushed and packed with special plastic bags of edible fungi with specifications of 18cm × 20cm, then sterilized by high pressure steam and inoculated. Take a small piece of Ganoderma lucidum cultivated species purchased to access the bacterial bag, and put it in a clean room for dark culture. The early room temperature is 24-26 ℃, the later room temperature is 22-24 ℃, and the indoor air relative humidity is 60%, 70%, to keep the air flowing. After about 40 days of culture, the mycelium was removed from the outdoor soil after it was full of mycelium. 2. Selection of cultivation site, construction of shed and bed: choose sandy loam with good drainage, ventilation and light transmission to build the field. Set up a "human" shaped shade shed, covered with plastic sheeting and covered with a shade net, it is better to take one-third of yang and seven percent of yin, and the light brightness is required to be uniform. The border runs from east to west, with a width of 1.3 meters, a length of 1.3 meters and a height of 30 centimeters. Soil disinfection was carried out in the border. 3. Burying the soil in the bacterial bag: when a few primordia appear in the bacterial bag and the temperature rises above 15 ℃, tear the plastic bag on the surface of the bacterial strip and arrange it upright on the finished border, the inoculation face up, the distance between the bacterial strips is 4-6 cm, the top of the bacterial bag is flat, fill the gap between the bacterial strips with fine soil, and the upper end is covered with soil of about 2 cm. Ganoderma lucidum management 1, regulation of light: Ganoderma lucidum fruiting body has higher requirements for light, and has a strong phototaxis. Too strong and weak light will cause conjoined or deformed cap, too strong will wither the young fruiting body, and too weak will make the stalk grow too long. Uneven light, or two-way light in the morning and afternoon, will cause the fruit body to tilt. Therefore, it is necessary to adjust the brightness of the shed to 400-600 lux candlelight and the light source is fixed. 2. Regulating temperature: Ganoderma lucidum is a medium-high temperature fungus, the growth temperature is 12.36 ℃, and the optimum temperature is 25-30 ℃. Close the shed door and cover the plastic film for heat preservation below 20 ℃; open the shed door for more than 30 ℃, open the surrounding plastic film, strengthen ventilation and cool down, and spray water on the roof to cool down. 3. Control humidity: Ganoderma lucidum likes humid environment. The water content of the artificial preparation medium was 60% ℃ 65%, and the fruiting body could differentiate into Ganoderma lucidum between 18 and 32 ℃, but the differentiation was the fastest and the development was the best at about 25 ℃. The most suitable air relative humidity for growth is 80% Murray 90%, and the soil moisture is about 50%. If the humidity is on the high side, ventilation should be used to reduce the humidity and stop watering in the border; if the humidity is on the low side, it is appropriate to spray into the space to improve the relative humidity of the air in the shed. 4. Improve ventilation conditions: Ganoderma lucidum is an aerobic fungus, the fruiting body is extremely sensitive to carbon dioxide, and the concentration of carbon dioxide in the air should be controlled below 0.03%. High levels will cause deformities. During the growth and development of the fruiting body, the door of the shed should be opened regularly, the surrounding plastic film should be opened, the ventilation condition should be improved and the concentration of carbon dioxide should be reduced. 5. In the cultivation of Ganoderma lucidum with mulberry branches, in order to reduce the production cost, mulberry branches can be used both as culture material and as fuel for sterilization.