Segment wood culture method of growing Ganoderma lucidum

For the selection and treatment of ① segment trees, broad-leaved trees such as oak, chestnut, tussah, peach, willow, poplar and Robinia pseudoacacia should be selected. The diameter is 5cm to 15cm. Immediately after the selection of tree species, cut down, but do not have to peel, saw grew into a 1-meter section of wood. If the moisture content of the tree species is higher, it should be piled up and dried until the wood wedge is used to enter the wood, and when the sap does not flow out, it can be inoculated. During the period of ② inoculation, the air temperature should be 20: 26 ℃ and the air relative humidity should be below 60%, which is beneficial to dry culture and avoid miscellaneous bacteria infection. After ③ inoculation, the fresh strains cultured for 20 days and the fruiting body primordium just formed were selected. This strain has strong vitality, develops rapidly after being connected to the wood, and is not afraid of miscellaneous bacteria infection. The inoculation tool can use a hole punch with a diameter of 1 to 1.2 cm, with a depth of 1 cm and a row spacing of 20 cm × 20 cm, staggered in the shape of "product". After drilling, inoculate immediately. Otherwise, the hole will dry and affect the survival rate. Vaccination should be carried out in a cool place. Before inoculation, take out the bacteria (cultivated species) for inoculation, cut them into small pieces of 1 square centimeter, gently plug them into the human hole, press them slightly, and cover them with wood wedges or bark. Then, flatten it gently with a small wooden hammer. About 30 days after inoculation, the hyphae invaded the wood, and brown bacteria circles were formed around the holes, indicating that the inoculation had survived. Then, the section of wood will be buried in the cultivation field for growth period management. ④ growth period management, after inoculation, the wood was framed into a "well" shape and dried for 10 to 15 days. Then, open the wood wedge or bark cover for observation, see that there is white mycelium, around the burrow becomes white or light yellow, and then gradually become light brown, wood wedge or bark cover has been covered with mycelium and fixed, that is to live. Immediately bury the wood in the acidic soil of pH5~6. If the weather is dry, the soil can be watered and moist.

In case of rainy season or rainy day, pay attention to drainage to avoid stagnant water in the site. In addition, it is necessary to sprinkle a circle of poisonous soil mixed with mirex around the cultivation ground to trap and kill termites to prevent harm. After Ching Ming Festival the following year, when the temperature rose to about 25 ℃, several pieces of wood could be taken out for inspection and opened the bark cover or wood wedge, and found that the hole had grown tea-brown around the hole, or had grown into a sesame bud, and there were white hyphae or beige mycelium membranes at both ends of the wood, and the special smell of Ganoderma lucidum hyphae could be smelled, indicating that the hyphae had matured. Dig out the wood immediately and cut off a small piece of wood that is 17cm long and 22cm long. Then, it is obliquely buried in the acid gravel soil, the upper end of the ground is exposed about 3 cm, and covered with weeds to cover the shade, drinks every few days to keep the soil moist, after 7 to 10 days began to grow Zhi Bud. During the period of growing Zhi Lai, the humidity in the cultivation ground was maintained at about 90%. If it is too dry and wet, it will lead to Zhi Lei's death. When cultured under suitable temperature and humidity, the sesame bud grew rapidly, first the stalk, and then the cap. It can be harvested in about two months. In the process of management, we should also pay attention to prevent miscellaneous bacteria infection, find Penicillium, Mucor, Rhizopus and other miscellaneous bacteria infection, you can use a burned blade to scrape off the surrounding bark and xylem, and then apply concentrated lime milk to prevent and cure, or use 75% alcohol cotton ball to fill the hole for prevention and treatment.