Formula of Pleurotus ostreatus strains how long is the production cycle of Pleurotus ostreatus
Maltose yeast Agar medium: 1 gram of soybean peptone, 2 grams of yeast, 20 grams of maltose and Agar, adding water to 1000 ml. Potato glucose yeast Agar medium: 300 grams of potato, 2 grams of yeast, 1 gram of peptone, 10 grams of glucose, 20 grams of Agar, adding water to 1000 ml. Oat flour maltose yeast Agar medium: oatmeal 80 grams, maltose 10 grams, yeast 2 grams, Agar 20 grams, add water to 1000 milliliters.
Pleurotus ostreatus is a rookie in edible mushrooms with bright color and high protein. It is a delicious food that integrates straw mushrooms, mushrooms and shiitake mushrooms. And Pleurotus ostreatus also has the effect of aiding digestion, preventing coronary heart disease and relieving mental fatigue. Friends eat more, but I believe you still do not understand the strain formula and production cycle of Streptomyces. Next, I will introduce it to you.
Formula of Pleurotus ostreatus
Pleurotus ostreatus has delicate taste, low production cost and can be cultivated artificially.
The method of strain production can be obtained by tissue isolation and spore isolation, which is basically consistent with the production method of mushroom and straw mushroom, while the new area mainly depends on the introduction of mother species or original species. The culture medium suitable for the production of Pleurotus ostreatus mother is as follows:
1. Mother medium
1. Maltose yeast Agar medium (MYA): soy peptone (soy peptone) 1g, yeast 2g, maltose 20g, Agar 20g, add water to 1000 ml.
2. Potato glucose yeast Agar medium (PDYA) 300g potato (add 1500 ml of water, boil for 20 minutes, filter juice), 2 g yeast, 1 g peptone, 10 g glucose, 20 g Agar, add water to 1000 ml.
3. Oat flour maltose yeast Agar medium (DMYA): oat powder 80g, maltose 10g, yeast 2g, Agar 20g, add water to 1000 ml.
If no Agar is added in the above three formulations, they can be used as liquid medium. The above culture media need to be routinely prepared, sub-packed, sterilized, inoculated and cultured.
2. Culture medium for original and cultivated species
1. Wheat straw or naked wheat straw, chopped (2mm long), soaked, bottled, autoclaved and set aside.
2. Soak wheat, rye, sorghum, corn, millet and other grains, boil thoroughly until there is no white core but the skin does not break, add 2% calcium carbonate, bottled and sterilized under high pressure.
3. 40% of sawdust and 40% of small wood chips and 20% of wheat bran to make culture medium.
The scraps of Pleurotus ostreatus or Flammulina velutipes can also be used as medium for re-sterilization.
III. Vaccination
It can be inoculated with liquid bacteria cultured for 4 days. If solid bacteria are used, the amount of inoculation must be increased (strain / culture material), at least 10%, preferably 15%, 20%.
IV. Training
After inoculation, put the strain bottle or bag in 20Mel 28 ℃ culture room. After the mycelium growth of Pleurotus ostreatus for a few days, the mycelium growth rate will gradually slow down, and the way to accelerate the mycelium growth is to stir. The aseptic wheat grain medium inoculated with liquid bacteria shakes the bottle every 3 seconds and breaks the mycelium, which can stimulate mycelium regeneration and ensure the exuberant growth of mycelium.
How long is the production cycle of Pleurotus ostreatus?
1. Land preparation: first of all, drainage ditches should be opened around the cultivation field, mainly to prevent stagnant water after rain.
2. Site disinfection: site disinfection should be carried out before the pile is built after the completion of land preparation.
3. Material treatment: the culture materials are used locally according to different regions, which are required to be fresh, dry and non-moldy. You can choose the following formula ① dry pure straw 100%. ② dry pure wheat straw 100%, ③ soybean straw 50%, corn straw 50%, ④ dried rice straw 80%, dry sawdust 20%, ⑤ dried rice straw 40%, grain husk 40%, miscellaneous sawdust 20%.
4. Pre-heap of straw soaking: the straw must absorb enough water before building the pile, and pre-fermentation is needed.
5. Composting bacterial bed: the most important thing of composting bacterial bed is to flatten the straw and increase the number of seeding holes, which can make the mycelium grow faster.
6. Cover mulch: add mulch to the haystack surface after construction and sowing, which can be covered with old sacks, non-woven fabrics, straw curtains, old newspapers and so on.
What is the market prospect of Pleurotus ostreatus
Streptomyces macrocephalus has a very broad development prospect, and it is distributed in Europe, North America, Asia and other places in nature. There are also cultivation and planting in many places in our country.
The cultivation of Pleurotus ostreatus has the advantages of rich raw materials, simple cultivation, strong stress resistance, wide temperature range and easy to succeed. The waste after cultivation can be directly returned to the field to improve the soil and increase fertility. And because of the long suitable season, it is beneficial to adjust the listing of other mushrooms or vegetables in the off-season. And Pleurotus ostreatus has high yield, low production cost and rich nutrition, so it is easily accepted by the majority of consumers.
However, the editor should also remind the vast number of mushroom farmers not to introduce unsuitable and inferior strains blindly, and not to deceive the vast number of growers for the sake of personal interests, by selling them strains that are not suitable for planting in this area.
Through the above, we have a certain understanding of the formula and production of Pleurotus ostreatus, whether stir-fried, fried, or soup, shabu-shabu, are very popular. Foreign countries are also very popular, growing big ball cover mushrooms is still very profitable.
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